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. 2017 Nov 17;8(66):110234–110256. doi: 10.18632/oncotarget.22622

Figure 2. M2 inhibits human gastric cancer cell line AGS migration and invasion.

Figure 2

(A-B) Wound-healing assay to study AGS cell migration at three different time (6, 12 and 24 hours) after treatment with 35μM M2. (C) Effect of M2 on invasion ability was measured using transwell assays in AGS cells. Microscopic observation (×10) of AGS cells on the bottom of the Boyden's chamber at the end of the 24 h. (D) Optical Density (OD) and percentage of cells which have invaded to the lower chamber. (E) effect of M2 on MMP activity analyzed by gelatin zymography. MMP-2 and MMP-9 activities were evidenced at the corresponding molecular weight (F) densitometric analysis of MMP-2 and MMP-9 activities. (n=3 biological replicates, error bars denote s.e.m.). *p value<0.005, relative to Control.