Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers |
Additional
information |
|---|---|---|---|---|
| recombinant DNA reagent | Salsa6f | Dong et al (doi: 10.7554/eLife. 32417) | ||
| strain, strain background (mouse) | Cd4-Salsa6f (Het), Cd4-Salsa6f (Hom) | Dong et al (doi: 10.7554/eLife. 32417) | ||
| strain, strain background (mouse) | NOD.Cg-PrkdcscidB2mtm1Unc/J (NOD.SCID.β2) | Jackson #002570 | ||
| strain, strain background (mouse) | NOD.CB17-Prkdcscid/J (NOD.SCID) | Jackson #001303 | ||
| strain, strain background (mouse) | Cd4Cre | Jackson #017336 | ||
| strain, strain background (mouse) | C57BL/6J | Jackson #000664 | ||
| transfected construct (synthetic) | eGFP-Orai1-E106A, eGFP-Orai1 | 23455504 | ||
| biological sample (human) | Primary T cells from healthy human subjects | UCI | IRB approved | |
| antibody | anti-MHC II (Clone Y3P), anti-MHC I (Clone AF6-88.5.5.3), IgG2a Isotype control (Clone: C1.18.4) |
BioXCell | ||
| antibody | anti-NK cell antibody | Wako Chemicals | ||
| antibody | αCD3 and αCD28 | Tonbo Biosciences | ||
| peptide, recombinant protein | recombinant human ICAM | R&D Systems | ||
| peptide, recombinant protein | recombinant human IL-2 | BioLegend | ||
| commercial assay or kit | EasySep human T Cell Isolation Kit | Stemcell Technologies | ||
| commercial assay or kit | EasySep mouse T Cell Isolation Kit | Stemcell Technologies | ||
| commercial assay or kit | Nucleofection kit | Lonza | ||
| commercial assay or kit | Sylgard Elastomer 184 kit | Dow Corning | ||
| chemical compound, drug | Cell tracker CMTMR, CellTrace Yellow or CellTrace Violet |
Life Technologies | ||
| software, algorithm | Imaris | Bitplane |