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. 2017 Dec 14;6:e32417. doi: 10.7554/eLife.32417

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© 2017, Dong et al

This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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Figure 4. — (A) Representative histogram showing cell trace violet (CTV) dilution in Cd4^Cre (dark grey), Cd4-Salsa6f (Het) (blue), and Cd4-Salsa6f (Hom) (red) T cells at 72 hr following stimulation with αCd3/28 Dynabeads (1:1 ratio). (B) Proliferation index measured on CTV dilution curves (n = 8). (C–E) Dot plots showing differentiation of naive T cells from Cd4^Cre and Cd4-Salsa6f (Het) mice into Th1 cells (C), Th17 cells (D) and iTregs (E) after 6 days (n = 4 mice). Right panels show average percentages of IFNγ⁺ cells (C), IL-17⁺ cells (D) and Foxp3⁺ cells (E).

Figure 4—figure supplement 1. — (A) Representative histogram showing cell trace violet (CTV) dilution in Cd4^Cre (dark grey), Cd4-Salsa6f (Het) (blue), and Cd4-Salsa6f (Hom) (red) T cells at 72 hr following stimulation with αCd3/28 Dynabeads (1:1 ratio). (B) Proliferation index measured on CTV dilution curves (n = 8). (C–E) Dot plots showing differentiation of naive T cells from Cd4^Cre and Cd4-Salsa6f (Het) mice into Th1 cells (C), Th17 cells (D) and iTregs (E) after 6 days (n = 4 mice). Right panels show average percentages of IFNγ⁺ cells (C), IL-17⁺ cells (D) and Foxp3⁺ cells (E).