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. Author manuscript; available in PMC: 2018 Oct 31.
Published in final edited form as: Biochemistry. 2017 Oct 19;56(43):5726–5738. doi: 10.1021/acs.biochem.7b00781

Figure 2.

Figure 2

Analytical HPLC traces (220 nm absorption) showing the speciation of S100A7 (9 μM) after different steps of the protein purification and following Cu(II)-catalyzed oxidation. The protein purification involves purification of S100A7 by AEC and SEC.27 Each trace was normalized to a maximum peak absorbance of 1. Analytical HPLC traces for S100A7Δ are presented in Figure S1 (Supporting Information). The shoulder peak observed in the chromatograms of S100A7ox corresponds to the isoform of S100A7 missing the N-terminal methionine.