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. Author manuscript; available in PMC: 2018 Jan 2.
Published in final edited form as: Biochemistry. 2016 Jul 6;55(28):3995–4002. doi: 10.1021/acs.biochem.6b00467

Table 1.

Nucleotide Inhibition Parameters for CVB3 3Dpol.

Globala
Graphical b
Wild Type Wild Type F364W I230F F232Y H228A H228E M310W
Kic(GTP) 590 ± 80 440 ± 60 490 ± 60 360 ± 80 460 ± 80 380 ± 90 350 ± 80 350 ± 75
Kic(UTP) 3100 ± 800 2900 ± 300 n.d.c n.d. n.d. n.d. n.d. n.d.
Kic(ATP) 1100 ± 200 1500 ± 200 1300 ± 100 800 ± 200 900 ± 200 1400 ± 200 1900 ± 300 1000 ± 110
Kiu(ATP) 1800 ± 100 1900 ± 300 2200 ± 400 n.a.d 3700 ± 900 n.a. 3900 ± 1000 2900 ± 400

All units are μM.

a

Global fitting with KinTek Explorer software with standard errors.

b

Graphical analysis via Dixon and Cornish-Bowden plots with standard error of the fitted parameters.

c

n.d. = UTP inhibition constants were not determined for the mutant polymerases.

d

n.a. = uncompetitive inhibition was not observed.