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. 2017 Dec 22;5(12):apps.1700100. doi: 10.3732/apps.1700100

Table 2.

Results of initial primer screening in populations of Primula species.a

P. ovalifolia P. tardiflora P. epilosa
OVA_EMS (n = 24) OVA_HZG (n = 24) OVA_BSH (n = 20) TAR_EMS (n = 24) EPI_PZ (n = 24)
Locus A Ho He A Ho He A Ho He A Ho He A Ho He
C11339 6 0.632 0.757 12 0.667 0.893 7 0.900 0.813* 3 0.136 0.210 1 0.000 0.000
C13959 4 0.313 0.619 3 0.250 0.507 3 0.056 0.517* 2 0.708 0.510 3 0.375 0.423
C14309 5 0.333 0.660 6 0.375 0.532 2 0.529 0.389 2 0.053 0.149 4 0.125 0.363*
C14710 6 0.833 0.779 7 0.708 0.764 8 0.857 0.847 3 0.813 0.679 3 0.565 0.569
C15228 5 0.579 0.731 5 0.300 0.278 3 0.300 0.296 — — — 1 0.000 0.000
C21614 3 0.231 0.495* 3 0.261 0.241 7 0.455 0.802 1 0.000 0.000 1 0.000 0.000
C23409 1 0.000 0.000 2 0.050 0.050 4 0.500 0.668 2 0.200 0.287 1 0.000 0.000
C23644 5 0.286 0.741* 12 0.739 0.887 4 0.842 0.698 3 0.667 0.519 2 0.083 0.082
C23746 1 0.000 0.000 2 0.125 0.120 2 0.000 0.278* 2 0.125 0.120 1 0.000 0.000
C24233 2 0.000 0.359 9 0.500 0.825* 3 0.049 0.050 2 0.375 0.311 2 0.261 0.232
C24268 10 0.500 0.870* 6 0.522 0.739 5 0.368 0.639* 6 0.429 0.606 1 0.000 0.000
C24676 3 0.087 0.405* 4 0.177 0.668* 4 0.938 0.666* 2 0.208 0.191 1 0.000 0.000
C40984 5 0.217 0.648* 2 0.895 0.508* 7 0.579 0.812* 6 0.333 0.559* 4 0.458 0.415
C45417 11 0.435 0.825 19 0.417 0.915 4 0.400 0.415* 6 0.250 0.509* 5 0.417 0.395
C46430 6 0.600 0.717 5 0.579 0.576 8 0.850 0.825 3 0.059 0.269 4 0.563 0.538
C47095 1 0.000 0.000 1 0.000 0.000 — — — 1 0.000 0.000 1 0.000 0.000
C48258 2 0.050 0.142 4 0.750 0.645 4 0.632 0.683* 1 0.000 0.000 4 0.652 0.654
C48475 5 0.278 0.819* 6 0.565 0.503 — — — 2 0.042 0.042 4 0.542 0.657
C50127 7 0.381 0.630 — — — 3 0.000 0.460* 3 0.750 0.573 4 0.318 0.698
C51170 6 0.750 0.761 11 0.542 0.872* 2 0.000 0.142* 3 0.476 0.441 — — —
C53509 11 0.632 0.871 6 0.263 0.713* 5 0.600 0.654 4 0.188 0.688* — — —
C53824 7 0.778 0.764 13 0.667 0.884 — — — 5 0.304 0.442* 2 0.000 0.089*
C53825 1 0.000 0.000 2 0.526 0.398 3 0.368 0.547 — — — 2 0.375 0.311
C53843 8 0.765 0.743 10 0.870 0.876 5 0.800 0.643 3 0.435 0.445 5 0.539 0.634
C53920 3 0.044 0.086* 2 0.117 0.156 1 0.000 0.000 3 0.435 0.530 2 0.042 0.042
C53962 5 0.273 0.459 4 0.368 0.647 1 0.000 0.000 3 0.167 0.519* 2 0.046 0.206*
C55683 2 0.000 0.502 9 0.478 0.857* 3 0.063 0.576* 6 0.350 0.782* 4 0.154 0.665*
C55722 2 0.235 0.371 4 0.364 0.444 4 0.200 0.595* 1 0.000 0.000 6 0.667 0.660
C57707 8 0.810 0.829 8 0.609 0.837 3 0.083 0.344* 2 0.143 0.143 3 0.208 0.196
C58140 10 0.500 0.808* 9 0.500 0.825* 2 0.050 0.049 3 0.792 0.543* — — —
C58437 3 0.684 0.522 2 0.067 0.067 2 0.600 0.495 2 0.478 0.476 — — —
C59012 2 0.053 0.053 3 0.191 0.180 3 0.200 0.184 3 0.762 0.638 1 0.000 0.000
C59078 2 0.263 0.309 — — — 1 0.000 0.000 3 0.385 0.631 2 0.048 0.048
C59155 3 0.046 0.09* 4 0.235 0.713 4 0.200 0.499* 1 0.000 0.000 1 0.000 0.000
C59702 6 0.563 0.794 6 0.409 0.538 3 0.111 0.106 4 0.200 0.407 2 0.208 0.311
C63472 4 0.154 0.542* 4 0.188 0.667* 5 0.375 0.734* 2 0.300 0.262 2 0.208 0.191
C65324 4 0.250 0.673* — — — 3 0.789 0.517 4 0.292 0.645* 5 0.478 0.728*
C66329 1 0.000 0.000 3 0.042 0.451 1 0.000 0.000 6 0.524 0.436 1 0.000 0.000
Meanb 5.2 0.389 0.587 6.1 0.421 0.582 4.0 0.400 0.510 3.3 0.367 0.437 3.2 0.306 0.379

Note: — = unsuccessful amplification; A = number of alleles; He = expected heterozygosity; Ho = observed heterozygosity; n = number of individuals sampled.

a

Locality and voucher information are provided in Appendix 1.

b

Monomorphic loci are excluded.

*

Significant deviation from Hardy–Weinberg equilibrium (P < 0.05).