There is an error in the first sentence of the “Protein Purification and Crystallization” section of the Materials and Methods. The correct sentence is: An N-terminal truncated form (residues 29−313) of the human pim1 gene was amplified using PCR and cloned into a modified pET28 vector (Novagen) using NdeI and NotI restriction sites, respectively.
Reference
- 1.Lee SJ, Han B-G, Cho J-W, Choi J-S, Lee J, Song H-J, et al. (2013) Crystal Structure of Pim1 Kinase in Complex with a Pyrido[4,3-D]Pyrimidine Derivative Suggests a Unique Binding Mode. PLoS ONE 8(7): e70358 https://doi.org/10.1371/journal.pone.0070358 [DOI] [PMC free article] [PubMed] [Google Scholar]
