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. 2017 Nov 26;8(65):108292–108302. doi: 10.18632/oncotarget.22660

Figure 1. SLPI is upregulated in highly metastatic cancer cells and promotes spontaneous lung metastasis in mice.

Figure 1

A. Comparison of secreted proteins in non-metastatic (67NR) and highly metastatic (4T1) cell conditioned media using a protein array that detects 350 mouse proteins. Arrows indicate secreted proteins markedly increased in highly metastatic cells including SLPI. B. Down-regulation of SLPI using shRNA in 4T1 cells inhibits lung metastases. The mice were implanted with control or SLPI-shRNA 4T1 tumors orthotopically and the lungs were examined for metastases (whitish nodules) 21 days after primary tumor resection. Lungs from mice pre-implanted with SLPI-shRNA tumors clearly exhibited fewer metastases (shSLPI). C. The number of macrometastases (colonies at least 0.5 mm in diameter) per mouse was significantly less in the mice bearing the SLPI-shRNA tumors (n = 7 for each group, *p = 0.004).