Skip to main content
. 2017 Jul 7;8(65):108430–108450. doi: 10.18632/oncotarget.19086

Figure 2. GSCs Proliferation assay and HIF-1α and PPARα and γ expression in GSCs.

Figure 2

A. BrdU assay in normoxia (N) and in hypoxia (H) of GSCs shows that hypoxic cells are more proliferating than normoxic ones.. Data are means ± SD of 3 different experiments. °°, p < 0.001, hypoxia vs normoxia. B. Western blotting analysis for HIF-1α, PPARα and γ in normoxic and hypoxic GSCs. In hypoxic conditions, a higher expression of HIF-1α and PPARα is observed, while PPARγ appears not significantly different. Data are means ± SD of 3 different experiments. °, p < 0.01, °°, p < 0.001, hypoxia vs normoxia. C. Immunofluorescence for HIF-1α, PPARα and γ in normoxic and hypoxic GSCs. In agreement with the western blotting results, HIF-1α and PPARα appeared highly expressed, mainly at nuclear level. Nuclei are stained with Dapi. Bar = 25µm.