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. 2017 Nov 24;8(65):109161–109174. doi: 10.18632/oncotarget.22644

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Copyright: © 2017 Dong et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License 3.0 (CC BY 3.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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(A) Apoptosis of different groups of NHME were measured using flow cytometry with double staining of Annexin V and PI. (B) Western blot of cytosol fraction of melanocytes demonstrated increase of Bax, cleaved-caspase 3 and cytochrome c protein levels and descrease of Bcl-2. Results were normalized against β-actin expression. (C) The band densities of interest proteins were measured by Tanon 5200 Multi analysis software program, and then the Bcl-2/Bax ratio were got. Results shown are means ± SD and are representative of 3 independent experiments. Data were analyzed by one-way analysis of variance (ANOVA) followed by post hoc Tukey test. ^**P < 0.01, compared with control.