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. 2017 Nov 25;8(65):109271–109288. doi: 10.18632/oncotarget.22669

Figure 4. AR can interact with ETS-1.

Figure 4

(A–B) The interaction of endogenous AR or ETS-1 with exogenous FLAG-ETS1 or FLAG-AR. FLAG-tagged AR (A), FLAG-tagged ETS-1 (B), or FLAG empty vector (A–B) was transfected into HepG2 cells. Cell lysates were immunoprecipitated by an anti-FLAG monoclonal antibody, and the precipitates were then immunoblotted with anti-ETS-1 or anti-AR antibody. (C–D) In vitro interaction between ETS-1 and AR. Glutathione-Sepharose beads bound with GST-AR (C), GST-ETS1 (D), or GST (C–D) were incubated with purified FLAG-labeled ETS-1 or AR in the presence or absence of 100 nM DHT. After washing the beads, the bound proteins were eluted and subjected to SDS-PAGE and IB assays.