Figure 2. Epitope cluster competition profiles with the V1C7 family of V_HHs.

Ricin was captured on microtiter plates by the surrogate receptor, asialofetuin (ASF; top row), or individual mAbs representing epitope clusters I–IV, as indicated by the vertical bars (left). Cluster III is defined by IB2 and cluster IV by GD12, as described ²⁰. The plates were then probed with V1C7 (gray), V2B9 (green), V2E8 (orange), or V5C1 (blue), as described in the Materials and Methods. The values shown are the average (with standard deviation) of a single representative experiment done in triplicate.