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The two VHH point mutants at position 29 were compared against their parental VHHs in a Vero cell cytotoxicity assay (see Materials and Methods). (Panel A) Ricin toxin-neutralizing activities associated with V1C7 versus V1C7G29R. (Panel B) Ricin toxin-neutralizing activities associated with V5C1 versus V5C1R29G. The values shown are the average (with standard deviation) of two separate experiments each done in triplicate.
