Fig. 3.

Overview of orthocaspases in various Microcystis aeruginosa strains and phylogenetic analysis of their p20 domains. a Overview of orthocaspases in all M. aeruginosa strains sequenced up to now. Strain and domain names can be found in Supplemental table (Online resource 1). Figure is not drawn to scale. The orthocaspase NIES-843 of M. aeruginosa is written in brackets to indicate its mutation of the cysteine residue in the specificity pocket (see also (c)). b Unrooted phylogenetic tree of all identified M. aeruginosa orthocaspases using the Neighbour-Joining method of MEGA software (version 6), based on their p20-like sequences aligned by PROMALS. NCBI identifiers can be found in Supplemental table (Online resource 1). Bootstrap values are shown on branches as percentages. Colours represent the active (dark blue) or putatively inactive (light blue) catalytic domain or the presence of additional domains C-terminal to the p20 domain with the same colour code as in a. c Sequence alignment of the specificity pocket in the region surrounding the cysteine residue. Identical residues are coloured black and similar amino acids are shaded in grey with 70% threshold for colouring. Conserved cysteine residues in the specificity pocket are marked in bold, their position is indicated by an arrow. The sequence alignment was performed using PROMALS and the figure was generated using BioEdit