FIG 2 .

Immunogenicity of recombinant ZIKV E protein in SW mice. (A) Vaccination schedule. The drawings of drops at days 14, 35, and 56 indicate blood draws. (B) Experimental groups. Alum, Imject alum. (C) Median fluorescence intensity (MFI) correlation of EC₅₀s with single-point MFIs. The log EC₅₀ values of seven different sera were determined by MIA. These values were plotted against the MFI values obtained from these sera at a dilution of 1:100 for each serum sample. (D) Geometric mean titers (GMTs) with 95% confidence intervals (CI) of ZIKV E-specific IgG MFI of sera from all groups of mice immunized with each formulation. S.D., standard deviations. *, P < 0.05; **, P < 0.01; ****, P < 0.0001.