Figure 10.

MAPK/ERK pathway inhibition abrogates enhanced granulosa cell proliferation following Jag1 knockdown. (A) Addition of 4 μM of MEK inhibitor PD98059 reduced the enhanced proliferation observed in siJag1 to that of the siScramble control. The baseline of granulosa cell proliferation in siScramble is unchanged by the addition of PD98059. (B) Inhibition of the AKT pathway using 10 nM of MK2206 did not impact proliferation in siRbpj or siScramble samples. n = 3; means not sharing the same letter are significantly different; P < 0.05.