Figure 4.

Notch ligands and receptors are expressed in cultured granulosa cells. Jag1 and Notch3 expression were found to be dynamically regulated throughout 7 days of granulosa cell culture. (A) Jag1 expression peaks at 3-day culture, whereas Notch3 expression continues to rise as the culture progresses. n = 3; *P ≤ 0.05 compared with 1-day culture. (B) The presence of JAG1 and NOTCH2 were confirmed using western blot. (C) Quantification of the western blot shows JAG1 levels decrease throughout the culture period beginning at day 5, whereas NOTCH2 levels transiently peak at day 5 of culture. n = 3, *P < 0.05 compared with 1-day culture.