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. 2017 Jun 9;158(9):1765–1779. doi: 10.1097/j.pain.0000000000000971

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Copyright © 2017 The Author(s). Published by Wolters Kluwer Health, Inc. on behalf of the International Association for the Study of Pain.

This is an open-access article distributed under the terms of the Creative Commons Attribution-Non Commercial-No Derivatives License 4.0 (CCBY-NC-ND), where it is permissible to download and share the work provided it is properly cited. The work cannot be changed in any way or used commercially without permission from the journal.

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Figure 3. — Analyses of cellular expression of miR-34c-5p in dorsal root ganglia in vivo. Representative images to demonstrate fluorescent in situ hybridization (FISH) analysis of miR-34c-5p expression in mouse DRG with specific or negative control probes (A) and immunofluorescence analysis of colabeling with its mRNA target Cav2.3 (A), isolectin‐B4‐binding (IB4) nonpeptidergic nociceptors (B), substance P‐positive peptidergic nociceptors (C) and NF200-positive large diameter sensory neurons (D) in mouse DRG. Cell nuclei were counterstained with DAPI. Quantification of coexpression of each neuronal subtype with the miR-34c-5p expressing neurons is shown in panel E. Scale bars represent 50 µm in all panels. Tissue samples from 3 independent mice were analyzed.