Author response image 1. FSC curves for an unpublished reconstruction obtained from a 300 kV Titan Krios.
Reconstructions calculated utilizing both frames 2-60 (green), corresponding to a cumulative dose of ~80 electrons, as well as frames 2-10 (red), corresponding to a cumulative dose of ~16 electrons/Å2, resulted in nearly identical resolution assessments of 3.6-3.7 Å. The full-dose reconstruction has more signal across all resolution shells, suggesting incorporating signal from more frames improves the map.