Figure 3. Metformin increased phosphorylation of AMPK and decreased phosphorylation of S6 in the OC cell lines.

The four OC cell lines were treated with metformin for 16 h. Protein was subsequently extracted, and western immunoblotting performed. Metformin induced expression of phosphorylated-AMPK and decreased expression of the phosphorylated-S6 protein. Results shown are one of three independent experiments.