Table 4. p53, BAX/BCL-2 ratio, cytochrome c mean fluorescence intensity of Jurkat cells after 24h, 48h and 72h treatment.
Jurkat | 6h | 24h | 48h | |||
---|---|---|---|---|---|---|
0 μM | 8μM | 0 μM | 8μM | 0 μM | 8μM | |
p53 | 214.7±1.2 | 238.2±0.9 | 341.9±1.7 | 400.9±0.2 | 590.7±1.6 | 608.2±1.4 |
24h | 48h | 72h | ||||
0 | 8μM | 0 | 8μM | 0 | 8μM | |
BAX/BCL-2 ratio | 1.1 | 0.8 | 0.9 | 0.7 | 0.9 | 0.4 |
Cytochrome | 33.7±0.8 | 35.8±1.3 | 32.3±1.3 | 34.5±0.5 | 35.9±0.6 | 29.8±0.8 |
Protein levels were evaluated by FCM as described in Methods. Data are presented as mean ± SEM of five independent experiments and analysed using the t-test for paired data.