Figure 3. Immunodetection of CD146 with radiolabeled TsCD146 mAb in cancer cells xenografted in nude mice.

(A) Fab’2-TsCD146 was radiolabeled with ⁶⁸Galium after NODAGA conjugation and injected in nude mice xenografted with C8161 melanoma cells. PET analysis showed that the tumor was detected with the radiolabeled antibody. Coronal, sagittal and axial views are shown. Tumors are located within dotted circles. Representative images of PET are shown. Experiments were performed with 6 different animals. (B) After sacrifice of the animals, tumors were labeled with TsCD146 mAb coupled to a FITC secondary antibody. A non-specific rat IgG was used as a control. The dotted line corresponds to the necrotic zone visualized in C. A representative image from 6 different experiments is shown. Magnification ×5. (C) After sacrifice of the animals, tumors were analyzed by histochemistry after treatment with eosin/hematoxylin. The dotted line corresponds to the necrotic zone. A representative image from 6 different animals is shown.