Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| cell line | HEK293T | ATCC | ATCC Cat# CRL-3216, RRID:CVCL_0063 |
|
| antibody | anti-FLAG M2 | Sigma-Aldrich | Sigma-Aldrich Cat# F1804, RRID:AB_262044 |
dilution: 1:1000 |
| c-Myc Tag Monoclonal Antibody (9E10) |
Thermo Fisher Scientific | Thermo Fisher Scientific Cat# MA1-980, RRID:AB_558470 |
dilution: 1:1000 | |
| Anti-beta III tublin Antibody |
Millpore | Millipore Cat# AB9354, RRID:AB_570918 |
dilution: 1:1000 in IF | |
| MAP2 | Millpore; PMID:26244549 | Millipore Cat# AB5622, RRID:AB_11213363 |
dilution: 1:1000 in IF | |
| actin | Millpore | Millipore Cat# MAB1501, RRID:AB_2223041 |
dilution: 1:2000 | |
| Tau-1 | Millpore | Millipore Cat# MAB3420, RRID:AB_94855 |
dilution: 1:1000 in IF | |
| Anti-Paxillin (N-Term), Rabbit Monoclonal, clone Y113 antibody |
Millpore | Millipore Cat# 04–581, RRID:AB_838293 |
dilution: 1:1000 in WB; 1:200 in IF |
|
| p-PaxillinY118 | ECM Biosciences | ECM Biosciences Cat# PP4501 | dilution: 1:1000 | |
| Mouse Anti-Paxillin Monoclonal Antibody, Unconjugated, Clone 349 |
BD Biosciences; PMID:28362576 | BD Biosciences Cat# 612405, RRID:AB_647289 |
dilution: 1:1000 | |
| Anti-Vinculin, clone V284 antibody |
millpore; PMID:28697342 | Millipore Cat# 05–386, RRID:AB_309711 |
dilution: 1:1000 in WB; 1:200 in IF |
|
| Anti-Integrin Beta1, activated, Clone HUTS-4, Azide Free antibody |
Millore; PMID:28602620 | Millipore Cat# MAB2079Z, RRID:AB_2233964 |
dilution: 1:1000 | |
| TRIP/CIP4 | Bethyl Laboratories; A301-186A | dilution: 1:1000 in WB; 1:200 in IF |
||
| Clathrin heavy chain antibody | Abcam; PMID:28231467, PMID:28575669 |
Abcam Cat# ab21679, RRID:AB_2083165 |
dilution: 1:1000 in WB; 1:200 in IF |
|
| Talin 1 and 2 antibody [8D4] | Abcam | Abcam Cat# ab11188, RRID:AB_297828 |
dilution: 1:1000 in WB | |
| Rabbit Anti-Rab5 Polyclonal Antibody, Unconjugated |
Abcam; PMID:28408870, PMID:28669519 |
Abcam Cat# ab18211, RRID:AB_470264 |
dilution: 1:1000 in WB | |
| Integrin beta 1 antibody [EP1041Y] - Carboxyterminal end |
Abcam; PMID:25330147, PMID:28552668, PMID:28609658 |
Abcam Cat# ab52971, RRID:AB_870695 |
dilution: 1:1000 in WB; 1:200 in IF |
|
| Phospho-FAK (Tyr397) Antibody (31H5L17), ABfinity(TM) Rabbit Monoclonal |
Thermo Fisher Scientific; PMID:22049075, PMID:25280968, PMID:26056143, PMID:26381152, PMID:26393679, PMID:26984758 |
Thermo Fisher Scientific Cat# 700255, RRID:AB_2532307 |
dilution: 1:1000 in WB; 1:200 in IF |
|
| Rabbit Anti-FAK [pY397] Polyclonal Antibodies, Unconjugated antibody |
Thermo Fisher Scientific; PMID:27474796, PMID:28069919, PMID:28520937 |
Thermo Fisher Scientific Cat# 44–624G, RRID:AB_2533701 |
dilution: 1:1000 in WB; 1:200 in IF |
|
| Akt1/2/3 (H-136) antibody | Santa Cruz Biotechnology; PMID:27316329, PMID:27410235, PMID:28911175 |
Santa Cruz Biotechnology Cat# sc-8312, RRID:AB_671714 |
dilution: 1:200 in WB | |
| Rac 1 (C-11) antibody | Santa Cruz Biotechnology | Santa Cruz Biotechnology Cat# sc-95, RRID:AB_2176125 |
dilution: 1:200 in WB | |
| Cdc42 (P1) antibody | PMID:28181299, PMID:28457749 | Santa Cruz Biotechnology Cat# sc-87, RRID:AB_631213 |
dilution: 1:200 in WB | |
| Rho A (26C4) antibody | Santa Cruz Biotechnology; PMID:28287395, PMID:28323616 |
Santa Cruz Biotechnology Cat# sc-418, RRID:AB_628218 |
dilution: 1:200 in WB | |
| Anti-Active Rac1-GTP Mouse Monoclonal Antibody |
NewEast Biosciences | NewEast Biosciences Cat# 26903, RRID:AB_1961793 |
dilution: 1:200 in WB and IF |
|
| Anti-RhoA antibody (mouse MAb)+control |
Cytoskeleton Inc; PMID:27822498 | Cytoskeleton Cat# ARH03, RRID:AB_10708069 |
dilution: 1:200 in WB and IF |
|
| Anti-Rac1 specific mouse MAb antibody |
Cytoskeleton Inc | Cytoskeleton Cat# ARC03-A, RRID:AB_10709099 |
dilution: 1:200 in WB and IF |
|
| Anti-Cdc42 Mouse Monoclonal Antibody |
Cytoskeleton Inc | Cytoskeleton Cat# ACD03-A, RRID:AB_10716593 |
dilution: 1:200 in WB and IF |
|
| peptide, recombinant protein |
PAK-GST, GST-Photekin-RBD | Cytoskeleton Inc | Cytoskeleton Inc Cat. #BK035; Cat. # BK036 |