LNCaP (A and B), LAPC-4 (C and D), VCaP (E and F) or C4-2 (H and I) cells were cultured in CM, SFM or CSM alone or CM, SFM or CSM with 1 nM T. Cell growth was measured on days 0 and 6 using MTT assay (OD590). Data were presented as mean +/− SEM. P-values for statistical comparisons of cell growth between Day 0 and Day 6 were listed in Supplemental Table S3. Comparisons among CaP cells cultured in CM, SFM or CSM alone or CM with T, SFM with T, CSM with T were listed in Supplemental Table S3.