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. 2017 Dec 15;144(24):4510–4521. doi: 10.1242/dev.152504

Fig. 6.

Fig. 6.

Effect of SOX9 overexpression in combination with ROCR depletion on MSC chondrogenic differentiation and gene expression. MSCs were transduced with SOX9 or control lentivirus (pCDH) for 1 day then transfected for 1 day with ROCR-targeting or non-targeting control (siCon) siRNA prior to chondrogenic differentiation in a V-bottom 96-well plate. (A) Expression of SOX9 in only non-targeting control siRNA pellets at Day 3. (B) GAG levels assayed by DMB assay in only non-targeting control siRNA pellets at Day 7. (C) Expression of SOX9 at Day 3. (D) GAG levels assayed by DMB assay at Day 7. (E,F) Expression of the indicated genes at Day 3. (C-F) Expression is presented as a percentage of non-targeting control levels for cells transduced with each virus. Values are mean±s.e.m. of data pooled from four MSC donors. *P<0.05; **P<0.01; ***P<0.001 for lncRNA siRNA versus non-targeting siRNA, or for SOX9 versus control lentivirus where indicated above the chart. Significant differences between sample groups were assessed by one-way analysis of variance followed by the Bonferroni post-hoc test for multiple comparisons or a two-tailed Student's t-test was performed for single comparisons.