Cadherin localization and cell adhesion at the four-cell-stage in the C. elegans embryo. (A) Micrographs of embryos expressing HMR-1 fused to GFP protein (LP172 strain) in untreated, hmr-1 (RNAi), par-2 (RNAi) and par-3 (RNAi) embryos, with or without eggshells. White arrowheads indicate cell–cell contact between EMS and P2 cells. Note that, for par-2 (RNAi) and par-3 (RNAi) embryos, the cell division orientations and cell arrangement patterns are variable and the micrographs presented here are examples (see also Fig. 8 for further examples showing the variability). Scale bars: 10 μm. (B) α of untreated, hmr-1 (RNAi), par-2 (RNAi) and par-3 (RNAi) embryos without eggshells at the four-cell stage. The box and whiskers are drawn as in Fig. 1C. ***P<0.001, Welch's t-test for ABd-ABd pair, ABd-EMS pair, and EMS-P2 pair.