Skip to main content
. Author manuscript; available in PMC: 2018 Feb 6.
Published in final edited form as: Oncogene. 2017 Oct 9;37(5):651–662. doi: 10.1038/onc.2017.372

Figure 2.

Figure 2

Reintroduction of exogenous eIF4E2 restores the ability of MDA-MB-231 cells to migrate, invade and adhere to one another in hypoxia. (a) Western blot of eIF4E2 protein levels in control (Ctrl) cells stably expressing a non-targeting shRNA, eIF4E2 knockdown control (KD Ctrl) cells stably expressing shRNA targeting eIF4E2 mRNA and an exogenous empty vector or the eIF4E2 coding sequence (Exo1 4E2 and Exo2 4E2). GAPDH used as a loading control. (b) KD Ctrl, Exo1 4E2 and Exo2 4E2 cells exposed to hypoxia (1% O2) for 24 h followed by wound generation. Representative images at 0 h (T0) and 16 h (T16) after wound generation. (c and d) Transwell migration (c) and invasion (d) assays of KD Ctrl, Exo1 4E2 and Exo2 4E2 cells exposed to hypoxia for 24 h. Representative images of transwell inserts 16 h after seeding and stained with crystal violet. (e) Light micrographs of spheroids composed of KD Ctrl, Exo1 4E2 and Exo2 4E2 cells. Data are presented as mean ±s.e.m., n ≥3, ***P<0.001, using a one-way ANOVA followed by Tukey’s HSD test. Scale bar, 100 μm.