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. 2018 Jan 16;9(1):e01845-17. doi: 10.1128/mBio.01845-17

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FIG 5 — Effect of overexpression of the pyoluteorin biosynthetic genes on the accumulation of spontaneous Gac⁻ mutants of P. protegens Pf-5. P. protegens Pf-5 derivative ΔpltA and pltR* ΔpltA strains were cultured in NYB. To test the expression level of pyoluteorin biosynthetic genes, plasmid ppltL-gfp containing a transcriptional fusion pltL::gfp, in which the promoter of pltL was fused with a promoterless gfp (11), was transformed into both strains, and the GFP level was assayed at 1 dpi (A). The accumulation of Gac⁻ mutants (B) and the total bacterial cell density (C) were recorded at 4 dpi. Each experiment had at least three replicates. Means ± standard deviations are shown.