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. 2017 Sep 27;43(3):607–616. doi: 10.1038/npp.2017.203

Oleic Acid in the Ventral Tegmental Area Inhibits Feeding, Food Reward, and Dopamine Tone

Cecile Hryhorczuk 1,2, Zhenyu Sheng 3, Léa Décarie-Spain 1,4, Nicolas Giguère 5, Charles Ducrot 5, Louis-Éric Trudeau 5, Vanessa H Routh 3, Thierry Alquier 1,6, Stephanie Fulton 1,7,*
PMCID: PMC5770761  PMID: 28857071

Abstract

Long-chain fatty acids (FAs) act centrally to decrease food intake and hepatic glucose production and alter hypothalamic neuronal activity in a manner that depends on FA type and cellular transport proteins. However, it is not known whether FAs are sensed by ventral tegmental area (VTA) dopamine (DA) neurons to control food-motivated behavior and DA neurotransmission. We investigated the impact of the monounsaturated FA oleate in the VTA on feeding, locomotion, food reward, and DA neuronal activity and DA neuron expression of FA-handling proteins and FA uptake. A single intra-VTA injection of oleate, but not of the saturated FA palmitate, decreased food intake and increased locomotor activity. Furthermore, intra-VTA oleate blunted the rewarding effects of high-fat/sugar food in an operant task and inhibited DA neuronal firing. Using sorted DA neuron preparations from TH-eGFP mice we found that DA neurons express FA transporter and binding proteins, and are capable of intracellular transport of long-chain FA. Finally, we demonstrate that a transporter blocker attenuates FA uptake into DA neurons and blocks the effects of intra-VTA oleate to decrease food-seeking and DA neuronal activity. Together, these results suggest that DA neurons detect FA and that oleate has actions in the VTA to suppress DA neuronal activity and food seeking following cellular incorporation. These findings highlight the capacity of DA neurons to act as metabolic sensors by responding not only to hormones but also to FA nutrient signals to modulate food-directed behavior.

Introduction

Long-chain fatty acids (FAs) are among the metabolic signals that are centrally detected to control energy homeostasis. Initial work discovered that hypothalamic neurons alter their firing rate in response to FA application (Oomura et al, 1975) while more recent studies demonstrated the impact of centrally administered FA to suppress feeding and hepatic glucose production (Lam et al, 2005; Obici et al, 2002; Schwinkendorf et al, 2011). The ability of FA to inhibit food intake and modulate autonomic function and neurotransmission is reported to be mediated by cellular FA transport and intracellular metabolism (Lam et al, 2005; Le Foll et al, 2013; Moulle et al, 2013; Obici et al, 2003). In turn, blocking FA hydrolysis from triacylglycerol in the brain has been shown to increase appetite and weight gain (Cansell et al, 2014; Picard et al, 2014b).

Several lines of evidence indicate that the influence of FA on energy metabolism depends on the chemical structure of the FA chain. The monounsaturated FA oleate (OA) and the saturated FA palmitate (PA) are the most abundant long-chain FAs in circulation and can exert different signaling and metabolic actions in the brain. OA, the most extensively produced FA in nature, has been shown to decrease food intake when acutely administered into the third ventricle (Obici et al, 2002; Schwinkendorf et al, 2011) and to inhibit or excite hypothalamic neurons (Jo et al, 2009; Le Foll et al, 2009; Wang et al, 2006). In addition, we have shown that OA has a distinct intracellular metabolic fate in neural cells as compared to PA (Taib et al, 2013).

The mesolimbic dopamine (DA) system has a key role in mobilizing behavior required to meet energy demands. DA neurons of the ventral tegmental area (VTA) respond to numerous hormones and peptides to modulate DA tone and food-directed behaviors (Fulton, 2010; Liu and Borgland, 2015). Prolonged intake of high-fat diets and the development of obesity can elicit molecular and neural adaptations in DA signaling that are associated with blunted reward responses and enhanced food seeking (Davis et al, 2008; Décarie-Spain et al, 2016; Fulton et al, 2006; Hryhorczuk et al, 2015; Johnson and Kenny, 2010; Stice et al, 2008; Wang et al, 2001). We recently demonstrated that long-term intake of dietary lipids enriched in PA (from palm oil) dampens mesolimbic DA function while a high-fat diet containing mostly OA (olive oil) is protective against such changes (Hryhorczuk et al, 2015). While these results suggest that the effects of chronic high-fat feeding on DA tone rely on the class of dietary lipids, it is not known whether FAs are detected by DA neurons and whether they can modulate appetite and mesolimbic DA neurotransmission. Here we report that intra-VTA injection of OA, but not PA, inhibited free-feeding intake and stimulated locomotor activity. VTA OA also blunted the rewarding effects of sucrose in an operant task and decreased DA neuronal firing amplitude and frequency of excitatory inputs onto DA neurons. DA neurons isolated from TH-eGFP mice were found to express genes encoding FA-handling proteins and we provide evidence for cellular transport of long-chain FA in DA neurons using a fluorescent FA analog. Finally, we demonstrate that a transporter blocker not only attenuates FA uptake into DA neurons but also blocks the effects of intra-VTA OA to suppress food-motivated responding and DA neuronal activity. Together, these findings suggest that FAs are detected by DA neurons and that the monounsaturated FA OA can suppress DA neuronal activity and the rewarding effects of food following cellular incorporation.

Materials and methods

Animals

Experiments were approved by the CRCHUM Animal Care Committee, the Université de Montréal Animal Ethics Committee, and the Institutional Animal Care and Use Committee of the New Jersey Medical School. All behavioral testing was carried out in rats to maintain precise VTA micro-injections, whereas remaining experiments were performed in mice due to the availability of transgenic lines and methods established for primary DA neuron cultures. Male Wistar rats (Charles River, Saint-Constant, Quebec) weighing 250–280 g upon arrival were housed in reverse-cycle rooms (lights off at 1000 hours) for 8–10 days before surgery. Male C57Bl/6 mice (4–6 weeks old) were used for electrophysiological recordings. TH-eGFP transgenic mice (C57Bl/6 background) expressing green fluorescent protein (GFP) under the control of the tyrosine hydroxylase (TH) promoter (Matsushita et al, 2002) were used for fluorescence-activated cell sorting (FACS). Primary DA neuron cultures were derived from postnatal (P0–P2) C57Bl/6 mice (Fasano et al, 2008).

Intra-VTA Injections

Stereotaxic surgery was carried out under isoflurane anesthesia and guided by rat brain atlas coordinates (Paxinos and Watson, 1998). Rats were implanted with bilateral guide cannula (26 gauge, Plastics One) terminating 2 mm above the VTA (5.8 mm posterior to the bregma, ±0.6 mm from midline, and −6 mm from dura). OA and PA (Na+; Sigma-Aldrich) were complexed to hydroxypropyl-β-cyclodextrin (HPB, CTD Holdings, Alachua, FL, USA) and dissolved in artificial cerebrospinal fluid (aCSF, in mM: 125 NaCl, 2.5 KCl, 0.3 KH2PO4, 26 NaHCO3, 10 glucose, 1.3 MgSO4, and 2.4 CaCl2; pH 7.4) to a final amount of 6 nmol per side. The 6 nmol dose represents 1/5 of that used in prior studies assessing feeding in response to third ventricle OA and PA complexed to HPB (30 nmol—Obici et al, 2002; Schwinkendorf et al, 2011). As we found a significant anorectic response to 60 nmol, but not 30 nmol, of OA infused into the lateral ventricle (data not shown) we used a 10:1 ventricle to parenchyma dilution to arrive at the 6 nmol intra-VTA dose. Phloretin (EMD Millipore) was solubilized in 15% ethanol and diluted to 100 μM in aCSF. Substances were delivered in a volume of 500 nl per side at a rate of 100 nl/min using a bilateral internal cannula (Plastics One) that projected 2 mm beyond the guide cannula.

Food Intake

Two cohorts of rats (total n=41) were used to evaluate the effect of intra-VTA OA and PA on chow intake. Rats were habituated to powder chow diet for 4–7 days. Food was removed 3 h before injection of OA or vehicle, or PA or vehicle, which occurred immediately preceding the onset of the dark cycle. Food cups filled with powder chow were fastened inside a larger cup to catch spillage. Food consumption and body weight were monitored at 2, 6, 12, and 21 h post injection.

Locomotor Activity

Ambulatory activity was evaluated in metabolic chambers (AccuScan Instruments, Columbus, OH, USA) in which rats were habituated 24 h before FA injection. Injections were done just before the onset of the dark phase.

Operant Responding

Another group of rats (n=10) with bilateral VTA cannulae were trained to respond for 45 mg high-fat plus high-sucrose pellets (TestDiet, Saint Louis, MO, USA) on a progressive ratio schedule of reinforcement in rat operant chambers with retractable levers (Med Associates, St. Albans, VT, USA) as described previously (Sharma et al, 2012). Once stable breakpoint responding was achieved, the effects of an intra-VTA vehicle injection was assessed followed by an injection of OA the following day. After breakpoint responding re-stabilized (number of rewards earned did not vary by more than 15%), operant responding was assessed in the same animals in response to vehicle and then OA+phloretin (100 μM in 0.5% ethanol) co-injection. Behavioral testing began 1 h after injection at the beginning of the dark phase. Cannula placement was verified by injections of methylene blue (500 nl per side) after completion of the experiment.

Electrophysiology

Whole-cell patch-clamp recordings were performed as described previously (Sheng et al, 2014). Briefly, 300 μm coronal slices containing the VTA were cut with a vibratome and maintained at room temperature for at least 1 h in oxygenated aCSF (in mM: 126 NaCl, 1.9 KCl, 1.2 KH2PO4, 26 NaHCO3, 2.5 glucose, 1.3 MgCl2, and 2.4 CaCl2; pH 7.4) before being transferred to the recording chamber. DA neurons were identified by the presence of an H current (Ih). Only cells with membrane potentials more negative than −45 mV in 2.5 mM glucose and with action potentials that overshoot 0 mV were used for recording. Only cells with an access resistance under 30 MΩ that did not vary by more than 20% throughout the experiment were kept for analysis. A total of 29 neurons were analyzed. Borosilicate pipettes (4–6 MΩ Sutter Instruments, Novato, CA) were filled with an intracellular solution containing (in mM): 128 K-gluconate, 10 KCl, 4 KOH, 10 HEPES, 4 MgCl2, 0.5 CaCl2, 5 EGTA, 2 Na2ATP, and 0.4 Na2GTP; pH 7.2. Cells with membrane potentials more negative than −45 mV in 2.5 mM glucose and action potentials, which overshoot 0 mV were considered viable for recording. Miniature excitatory postsynaptic currents (mEPSCs) were recorded in neurons voltage-clamped at −60 mV in extracellular solution supplemented with 1 μM tetrodotoxin (TTX, to block presynaptic action potentials) and 20 μM bicuculline (to block GABAA currents). A unit of 6 μM OA was bath perfused for 10 min. In the transporter blockade condition, 100 μM phloretin was co-applied with OA for an additional 10 min. Recordings from the last minute of each condition were used in the analysis.

Fluorescence-Activated Cell Sorting

P0–P2 mice were cryo-anesthetized and decapitated for tissue collection. As described previously (Fulton et al, 2011; Mendez et al, 2008), freshly dissociated cells from the VTA were prepared and GFP-positive neurons were purified by FACS and directly collected in Trizol (Qiagen, Toronto, ON, Canada).

Quantitative PCR

Total RNA from VTA and ventromedial hypothalamus (VMH) microdissections and FACS-sorted cells were extracted using Trizol. Concentration and purity of RNA were systematically assessed. RNA were reverse-transcribed into cDNA using the Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT, Life Technologies, Burlington, ON, USA). Genes were amplified using the Rotor-Gene SYBR Green PCR Kit (Qiagen) in the presence of appropriate primer pairs. Results were normalized to β-actin or cyclophilin and analyzed using the standard curve method (Taib et al, 2013).

Primers

Primers were designed using BLAST (U.S. National Library of Medicine) and synthesized by Integrated DNA Technologies based on the following sequences: actb (β-actin) forward: 5-′TTCTTGGGTATGGAATCCTGTGGCA-3′, reverse: 5′-ACCAGACAGCACTGTGTTGGCATA-3′ cd36 forward: 5′-TGCATGAATTAGTTGAACCAGGCCA-3′, reverse: CCACAGTTCCGATCGCAGCC-3′, ppia (cyclophilin) forward: 5′-GCTTTTCGCCGCTTGCTGCA-3′, reverse: TGCAAACAGCTCGAAGGAGACGC-3′ fabp3 forward: 5′-GATGACCGGAAGGTCAAGTC-3′, reverse: 5′-GCCATGAGTGAGAGTCAGGA-3′ fabp5 forward: 5′-AAACCGAGAGCACAGTGAAGA-3′, reverse: 5′-AAGGTGCAGACCGTCTCAGT-3′ fabp7 forward: 5′-AGTACATGAAGGCTCTGGGCGTG-3′, reverse: 5′-ATCACCACTTTGCCGCCTTCCT-3′ fatp1 forward: 5′-GCAGGTACTACCGCATTGCT-3′, reverse: 5′-GAACTTCTTGCGCAGTACCA-3′ fatp4 forward: 5′-TGTGGTGCACAGCAGGTATT-3′, reverse: 5′-TTTCCTGCTGAGTGGTAGAGG-3′ gad1 (GAD67) forward: 5′-ATATCATTGGTTTAGCTGGTGAATG-3′, reverse: 5′-GTGACTGTGTTCTGAGGTGAAGAG-3′ th forward: 5′-CGACCCGTGGCCGGTCTAC-3′, reverse: 5′-GCAGTCTGGCTCGGGTGAGTG-3′.

FA Uptake in DA Neurons

Midbrain DA neurons grown on a cortical glial layer were prepared from P0–P2 wild-type mice as described (Fasano et al, 2008). FA uptake was assessed with C1-BODIPY 500/510-C12, a fluorescent FA analog that with the fluorophore head resembles the chemical structure of a long-chain FA (20 μM in 0.8% DMSO; ThermoFisher). Ten days after seeding, cells were treated with BODIPY, BODIPY+phloretin (100 μM in 0.5% ethanol), or vehicle alone in culture medium (Neurobasal additioned with glutamax) for 45 min. Following three washes with ice-cold PBS cells were fixed with 10% formalin for 10 min. TH immunofluorescence was carried out as described previously (Fulton et al, 2011). The ImageJ analyzing tool (http://imagej.nih.gov/ij/) was used to determine BODIPY fluorescence intensity. For each condition, integrated density was measured in × 63 magnified fields (n=45) derived from three individual coverslips per condition.

Statistical Analyses

Data are presented as mean±SEM and were analyzed using GraphPad Prism (version 5.02 for Windows GraphPad Software, San Diego, CA, USA). Two-way analysis of variance (ANOVA) with Bonferonni post-tests was used to analyze food intake and locomotor activity data. One-way ANOVA was used in electrophysiology and immunofluorescence with Bonferonni post-tests. Operant responding and quantitative PCR (qPCR) data were analyzed with Student’s t-test. Statistical significance was set at p⩽0.05.

Results

Intra-VTA Oleate Inhibits Food Intake and Stimulates Locomotor Activity

We tested the acute effects of intra-VTA OA and PA on food intake. As illustrated in Figure 1a and b, OA significantly inhibited chow intake 12 h after injection (vehicle: 23.9±1.3 g, OA: 19.6±1.6 g; 18% decrease; repeated measures two-way ANOVA; F1,21=4.47, p<0.05) while stimulating ambulatory locomotor activity (F1,12=5.55, p<0.05). Comparatively, an equimolar dose of PA in a different cohort of animals did not alter chow intake (F1,14=0.46, p>0.05) or locomotion (F1,16=0.07, p>0.05; Figure 1c and d). Bilateral VTA cannula coordinates were confirmed by rhodamine microbead injections (Supplementary Figure S1A). The anorectic effects of OA or PA were not associated with local inflammation as IL-1β and TNFα mRNA levels in the VTA were unchanged following OA injection (OA: IL-1β: t17=0.29; TNFα: t17=0.35; PA: IL-1β: t4=0.84; TNFα: t4=1.25, p>0.05; Supplementary Figure S1B and C).

Figure 1.

Figure 1

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Intra-VTA oleate inhibits food intake and stimulates locomotor activity. (a, b) Bilateral micro-injection of OA (6 nmol/side) reduced food intake (a) and increased locomotor activity (b). (c, d) Injection of PA into the VTA at an equivalent concentration had no effect on food intake (c) and locomotion (d). n=8–13/group for food intake assessments; n=7–10/group for locomotor activity experiment. Results are expressed as mean±SEM. Two-way repeated measures ANOVA, *p<0.05 vs vehicle-injected rats.

Intra-VTA Oleate Suppresses Food Reward

To determine whether OA alters motivation for palatable food we assessed effort-based, operant responding for high-fat/high-sugar food rewards in a progressive ratio task. OA decreased breakpoint thresholds by 23% relative to vehicle treatment (t7=2.56, p<0.05; Figure 2a). As the central effects of FA can involve transporter-mediated processes, we next sought to establish whether blocking intracellular uptake could mitigate the ability of OA to suppress food-motivated behavior. The injection of the transporter blocker phloretin (100 μM/side) alone did not alter chow food intake (Supplementary Figure S2; F1,13=0.59, p>0.05) or high-fat/high-sugar pellets responding (Supplementary Figure S3; t5=1.05, p>0.05). It did however prevent the attenuated breakpoint responding induced by OA (t6=0.31, p>0.05; Figure 2b). No changes were observed in the proportion of correct to incorrect lever presses (OA: t7=0.27; OA+phloretin: t6=0.66, p>0.05; Figure 2c and d). Subsequent histological assessment revealed that bilateral cannulas were positioned in the VTA in all rats (Figure 2e).

Figure 2.

Figure 2

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Oleate blunts the rewarding effects of high-fat/-sugar food. (a) Intra-VTA injection of OA decreased breakpoint responding for high-fat/-sucrose pellets in a progressive ratio operant task. (b) The co-injection of the transporter blocker phloretin prevented the effect of OA. (c, d) The percentage of correct lever responses was unaffected by either treatment. (e) Injector cannula placements; each symbol depicts a different rat (n=7). Results are expressed as mean±SEM. *p<0.05.

Oleate Modulates DA Neuronal Activity

Whole-cell patch recordings were performed to establish whether reductions in food intake and food reward by intra-VTA OA are associated with changes in the activity of DA neurons. Current-clamp recordings revealed an effect of OA to inhibit DA neuronal activity: 52% of neurons (15/29) decreased their action potential frequency (APF) in response to OA (basal: 0.91±0.09 Hz vs OA: 0.44±0.07 Hz). We next ascertained whether OA-induced changes rely on intracellular incorporation of OA by co-applying phloretin and OA during current-clamp recordings. Phloretin blocked the effect of OA to inhibit APF (OA: 0.44±0.07 Hz vs OA+phloretin: 0.75±0.08 Hz; F2,14=8.16, p⩽0.001; Figure 3a and b, representative trace). We then examined the influence of OA on mEPSCs (in presence of TTX). OA significantly decreased current amplitude (basal: 10.29±1.48 pA vs OA: 6.25±1.03 pA) and frequency (basal: 1.69±0.19 Hz vs OA: 1.13±0.11 Hz), an effect that was partially reversed by co-application of phloretin (amplitude: OA, 6.25±1.03 pA vs OA+phloretin, 8.71±1.49 pA; frequency: OA, 1.13±0.11 Hz vs OA+phloretin, 1.4±0.15 Hz; amplitude: F2,6=44.79, p⩽0.0001; frequency: F2,6=25.08, p⩽0.0001; Figure 3c–e, representative trace). A smaller subset of DA neurons (9/29) exhibited an increase in APF in response to OA (basal: 1.01±0.28 Hz vs OA: 1.38±0.32 Hz); however, unlike with OA-inhibited neurons, phloretin had no effect on APF in OA-excited neurons (OA: 1.38±0.32 Hz vs OA+phloretin: 1.29±0.29 Hz; F2,8=26.04, p<0.0001). Remaining neurons (5/29) were unresponsive to OA (basal: 1.84±0.74 Hz, OA: 1.65±0.59 Hz; F2,4=0.55, p>0.05).

Figure 3.

Figure 3

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Oleate modulates dopamine neuronal activity. (a) Whole-cell current-clamp recordings of spontaneous electrical activity of VTA DA neurons. OA decreased action potential frequency (APF) of the majority of recorded DA neurons. The co-application of phloretin prevented OA-induced APF inhibition. n=6. (b) Representative trace. Resting membrane potential is indicated by the horizontal dashed line and noted on the left. (c, d) Voltage clamp recordings showing that OA decreased the amplitude (ImEPSC, c) and the frequency (d) of miniature excitatory post-synaptic currents in OA-inhibited neurons, an effect prevented by phloretin. n=7. (e) Representative traces. Downward deflections represent mEPSCs. Results are expressed as mean±SEM. One-way standard (a) or repeated measures (c, d) ANOVA. *p<0.05; **p<0.01; ***p<0.001 vs basal condition; #p<0.05; ###p<0.001 vs OA condition.

DA Neurons Express FA-Handling Proteins

Specific isoforms of FA transport proteins (FATP) and FA-binding proteins (FABP) are centrally expressed, namely CD36, FATP1, FATP4, FABP3, FABP5, and FABP7 (Liu et al, 2010; Mitchell et al, 2011b). To determine whether these FA-handling proteins are present in DA neurons we performed qPCR experiments in FACS-purified GFP positive (GFP+) and negative (GFP−) cells isolated from the VTA of TH-eGFP mice and compared to expression levels obtained from VTA and VMH microdissections. Given that the metabolic and neurophysiologic actions of FA have been most characterized in the VMH all data were normalized to results obtained from this region. That sorted GFP+ cells were dopaminergic is evidenced by high expression of TH and absence of GAD67 (GABA marker). In contrast, there was minimal TH in GFP− cells. DA neurons and the VTA as a whole were found to express FATP1, FATP4, and FABP3, with minimal expression of CD36, FABP5, and FABP7 (Figure 4).

Figure 4.

Figure 4

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Gene expression profile of fatty acid-handling proteins in dopamine neurons and VTA. DA neurons (GFP+) and non-DA cells (GFP−) were FACS-sorted from TH-eGFP mice. GFP+ neurons were dopaminergic as evidenced by high levels of TH mRNA and absence of GAD67 mRNA. Cyclophilin served as reference gene and all results (except for TH and GAD67) were normalized to values obtained from VMH tissue. FABP, fatty acid-binding protein; FATP, fatty acid-transport protein; GAD67, glutamate decarboxylase; TH, tyrosine hydroxylase.

FAs are Taken Up by DA Neurons

FA intracellular transport was assessed with BODIPY, a fluorescent long-chain FA analog. BODIPY application to primary DA neurons resulted in punctate labeling in TH immunopositive (TH+) neurons as well as labeling in non-TH cells (Figure 5e, magnified in 5h). Optical Z-stack imaging and orthogonal reconstruction revealed cellular BODIPY labeling of spherical form resembling lipid droplets in TH+ cell bodies and processes (Figure 5e and f). The magnitude of BODIPY incorporation was diminished by the addition of phloretin to the incubation medium (Figure 5f and g). BODIPY application significantly increased cellular fluorescence intensity (expressed as integrated density value) compared to the vehicle condition, whereas co-application of phloretin attenuated the intensity of fluorescent labeling in TH+ neurons (F2,44=502.0, p⩽0.0001; Figure 5j).

Figure 5.

Figure 5

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Fatty acid uptake in primary dopamine neurons. Application of the long-chain FA analog BODIPY to the culture medium resulted in intracellular punctuate labeling (green) in the cytoplasm as well as processes of DA neurons identified by TH immunofluorescence (red). (a, d, g) Vehicle application. (b, e, h) BODIPY (20 μM) application. (c, f, i) BODIPY (20 μM)+phloretin (100 μM) application. (j) BODIPY fluorescence intensity values across treatment conditions. Three coverslips per condition. Results are expressed as mean±SEM. One-way ANOVA. ***p<0.001 vs vehicle; ###p<0.001 vs BODIPY alone. TH, tyrosine hydroxylase.

Discussion

Central lipid sensing contributes to the control of food intake and energy homeostasis, whereas impediments in this process can dispose to weight gain and obesity. As initial evidence that FA can be detected in the midbrain, the present study reveals that OA suppresses feeding and food reward, and stimulates locomotor activity upon acute administration into the VTA. Shedding light on the cellular mechanisms involved, we demonstrate that OA can inhibit DA neuronal firing, that DA neurons comprise the machinery for FA uptake and shuttling, that long-chain FAs are taken up by DA neurons, and that blocking cellular transport counteracts the behavioral and electrophysiological effects of OA in the VTA. These findings underscore the capacity of DA neurons to act as metabolic sensors by responding not only to hormones but also to FA nutrient signals to generate adaptive changes in feeding and food-directed behavior.

We chose the intra-VTA study design to determine the effects of FA specifically in the VTA. While it is not yet clear whether circulating FAs reach the VTA we believe it is likely they do given that (1) manipulation of triglyceride hydrolysis into free FAs (knockout of lipoprotein lipase) in extra-hypothalamic sites such as the nucleus accumbens (NAc) (Cansell et al, 2014) and hippocampus (Picard et al, 2014b) alters feeding behavior; (2) lipoprotein lipase is enriched in the VTA, at concentrations that are higher than other brain regions (Chung et al, 2005; Greene et al, 2005; Grimm et al, 2004); (3) as demonstrated in the present work, FA transporters and binding proteins are expressed in the VTA, in dopaminergic and non-dopaminergic cells; and (4) multiple metabolic signals (insulin, leptin, ghrelin, GLP-1, and amylin) that target the hypothalamus also have signaling actions in the VTA. While the physiological levels of FA in the VTA are unknown, the 6 nmol dose was extrapolated from a combination of previous reports (Obici et al, 2002; Ross et al, 2010; Schwinkendorf et al, 2011) and our own experiments testing ICV oleate on food intake. Despite a more physiological route to the VTA, intravenous or intra-gastric administration would not permit establishing whether FAs are detected by the VTA to modulate behavior. Indeed, an intra-gastric infusion of FA would modulate secretion of various incretin hormones such as GLP-1, stimulate the enteric nervous system, and modulate oleoylethanolamides, each of which can affect the mesolimbic DA system (Fortin et al, 2016; Tellez et al, 2013). Similarly, an i.v. infusion of FA would stimulate insulin secretion, which is also known to target VTA DA neurons (Labouebe et al, 2013). The anorectic influence of intra-VTA OA observed is consistent with its actions to decrease food intake when delivered into the third ventricle (Obici et al, 2002; Schwinkendorf et al, 2011). Moreover, our results distinguish the impact of OA by demonstrating that the same dose of PA, another ubiquitous long-chain FA, fails to affect food consumption when administered into the VTA. We cannot exclude, however, that a higher concentration of PA might affect feeding. Importantly, the infusion of either FA was not associated with local toxicity and inflammation. These findings are in line with previous evidence that unsaturated FA species (OA and docosahexanoic acid), but not saturated FA like PA, have an anorectic action upon intraventricular infusion (Schwinkendorf et al, 2011). PA has been more associated with inflammatory pathway stimulation and diminished central leptin and insulin signaling (Benoit et al, 2009; Kleinridders et al, 2009). Concomitant with reduced feeding, rats exhibited an increase in locomotor activity following OA (but not PA) injection, an effect that could be due to increased time available for behaviors other than feeding and/or to direct cellular actions of OA in the VTA. Although others have reported opposite effects of OA on locomotion (Contreras et al, 2014; Gobbi et al, 1999), the peripheral route of administration in these studies may well explain the discrepancy with our results.

As the VTA and its corticolimbic targets are a critical component of the circuitry controlling motivation, we investigated the effect of OA on effort-based responding for palatable food. Acute OA into the VTA diminished the rewarding effects of high-fat/-sucrose food. This finding is in agreement with previous work demonstrating that intracarotid infusions of a lipid emulsion (intralipid—comprising mostly polyunsaturated and monounsaturated FAs) reduces food-motivated responding (Cansell et al, 2014). Moreover, Cansell and colleagues demonstrated that deletion of the gene encoding lipoprotein lipase, an enzyme hydrolyzing FA from triacylglycerol, from the NAc blocks the effects of lipid infusion to decrease food reward. It remains to be determined whether the effect of moderating FA levels in the NAc on food-seeking is mediated by distinct FA such as OA and which mechanisms are involved.

In accordance with blunted motivation and food seeking, the firing frequency of the majority of recorded DA neurons was reduced in response to OA application. As has been demonstrated with other anorectic hormones and peptides (Liu and Borgland, 2015), OA can directly inhibit DA neuronal activity thereby bypassing FA-sensing mechanisms of the hypothalamus. Similar to observations in the hypothalamus (Jo et al, 2009; Wang et al, 2006), ~80% of DA neurons responded to OA, 52% were inhibited, and 29% were excited by OA. While this possibility remains to be tested, DA neurons excited by OA could be involved in the locomotor-enhancing effect of intra-VTA OA injection. As we previously showed, a subset of leptin-responsive DA neurons in the VTA modulates locomotor activity and running reward (Fernandes et al, 2015) and thus perhaps oleate may have similar actions by stimulating the activity of these neurons. These divergent neuronal activity changes in response to OA suggest that DA neurons can be distinguished based on their response to nutritional signals. Indeed, heterogeneity in the functional, anatomic, electrophysiological, and genetic properties of VTA DA neurons is well documented (Morales and Margolis, 2017). In this regard it is important to mention that as we used rats and mice for our experiments, differences in the activity of VTA DA neurons across species may limit the interpretation of our findings. OA treatment had no effect on membrane potential or input resistance suggesting that it does not modulate non-synaptic (background or leak) ion channel closure/opening. OA significantly reduced the frequency of mEPSCs indicating a reduction in the probability of presynaptic glutamate release. The decrease in mEPSC amplitude may reflect this decrease in transmitter release; however, it could also suggest post-synaptic modifications of ionotropic glutamate receptors.

FATPs and FABPs are centrally expressed and contribute to cell differentiation, FA uptake, energy balance, and cognitive processes (Moulle et al, 2012). While there is de novo FA synthesis in the brain (Medina and Tabernero, 2002), the FAs incorporated in neural cells are largely derived from the periphery. Our results demonstrate that DA neurons express the machinery for intracellular FA transport and shuttling, and that they are capable of FA incorporation. Interestingly, expression of CD36, the FATP most tied to the effects of intra-hypothalamic FA on energy balance (Le Foll et al, 2013; Moulle et al, 2013), was minimal or absent in the VTA and in purified DA neurons, respectively. Rather, FATP1 and FATP4 were expressed at relatively elevated levels and thus could participate in cellular uptake and behavioral actions of OA in VTA. Among FA-binding proteins expressed in brain, FABP5 is predominantly expressed in immature neurons and glia, FABP7 in glial cells, and FABP3 is enriched in mature neurons (Mitchell and Hatch, 2011a). Correspondingly, DA neurons exhibited high levels of FABP3 (compared to whole VTA and VMH) and minimal FABP5 and FABP7 expression, a finding consistent with reports of FABP3 expression in substantia nigra DA neurons in mice (Shioda et al, 2014). FABP3 also exhibits considerable affinity for monounsaturated FA such as OA (Tan et al, 2014) to further suggest a potential role for FABP3 in mediating the effect of OA in DA neurons. Along with the expression of genes encoding FA-handling proteins we found that DA neurons are capable of intracellular FA transport. While BODIPY FA is not a perfect analog for oleate, it is processed by cells as a long-chain FA (Carten et al, 2011) and is readily taken up by neurons (Kaushik et al, 2011). BODIPY accumulated in structures resembling lipid droplets, which are intracellular organelles that store triglycerides. This observation is consistent with our previous work showing that OA is esterified into triglycerides in hypothalamic neurons (Taib et al, 2013) and suggests that the metabolic fate of FA is similar between VTA DA and hypothalamic neurons.

The mechanisms by which FA gain entry into cells are not entirely clear, however several lines of evidence implicate active transporter-mediated processes (Mitchell and Hatch, 2011a). With the application of phloretin, a nonspecific inhibitor of FA transport (Chabowski et al, 2013), we show that blocking transport diminishes FA uptake into DA neurons and prevents OA-induced suppression of food-motivated responding and DA neuronal activity. Along with the gene expression profile, these results suggest that the behavioral and physiological actions of intra-VTA OA at least partly rely on interactions with transporters and/or binding proteins. In the hypothalamus, the effects of FA on neuronal activity and feeding are largely tied to the membrane FA transporter CD36 and are shown to be independent of FA intracellular metabolism (Le Foll et al, 2013; Moulle et al, 2013). However, FA-induced alterations in ion channel activity as well as FA mitochondrial oxidation can also account for the cellular effects of FA (reviewed in Picard et al, 2014a). Such alternative pathways may be involved in the actions of OA observed here as CD36 expression was barely detectable in DA neurons and VTA. Although our FA uptake and electrophysiological data support a direct effect of OA on DA neurons, we cannot exclude the possibility that OA modulates DA neurons via regulation of GABA inputs or neighboring astrocytes. Indeed, astrocytes take up, metabolize, and release FA (Bouyakdan et al, 2015; Taib et al, 2013) and FA oxidation in astrocytes produces ketone bodies (KBs) (Bixel and Hamprecht, 1995) that could in turn alter neuronal activity (Guzman and Blazquez, 2001; Ma et al, 2007). Moreover, Le Foll et al (2014) describe that hypothalamic KB generation induced by high-fat feeding decreases food intake. Thus, we cannot rule out the contribution of FA-derived KB and look toward future investigations to identify the metabolic and molecular signals involved in the impact of OA in the VTA.

Conclusion

The collective results suggest that OA in the VTA represents a signal of energy surfeit to curb food intake and food-oriented behaviors. That these catabolic actions were selective to a monounsaturated but not saturated long-chain FA further emphasizes the significance of FA type in the central signaling and metabolic action of FA. The effects of OA to suppress appetite and food-motivated behavior were linked to OA-induced inhibition of DA neuronal firing and to interactions with FA transporters, possibly including intracellular OA uptake. Brain FA content is fairly stable in response to short-term dietary changes in FA; however, human obesity and the metabolic syndrome are linked to increased central FA uptake (Karmi et al, 2010). Thus, a question for future research is whether VTA sensing of FA is altered by prolonged consumption of energy-dense food and the development of obesity, and whether such changes could contribute to uncontrolled reward-seeking and/or hyperphagia and weight gain.

Funding and disclosure

The authors declare no conflict of interest.

Acknowledgments

We thank Marie-Josée Bourque for neuron cultures and help with cell sorting, and Demetra Rodaros and Anna Kristyna Franco Flores for helping with intra-VTA surgeries and operant responding experiments. This work was supported by a CIHR grant (MOP123280) and New Investigator salary award to SF, by a CIHR grant (MOP115042) and Fonds de Recherche Québec-Santé salary award to TA, by CIHR (MOP106556) and Brain Canada/Krembil Foundation grants to L-ET, American Heart Association grants to VHR (14GRNT20380639 and 1RO1DK103676), and doctoral scholarships from the Montreal Diabetes Research Center/Université de Montréal and CMDO/Novo Nordisk to CH.

Footnotes

Supplementary Information accompanies the paper on the Neuropsychopharmacology website (http://www.nature.com/npp)

Supplementary Material

Supplementary Information
Click here for additional data file. (393.5KB, pdf)

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