ABSTRACT
We report here the draft genome of Cobetia sp. QF-1, a cold-adapted bacterium isolated from crude oil-contaminated seawater of the Yellow Sea, China. This genome is approximately 4.1 Mb (G+C content, 57.44%) with 3,513 protein-coding sequences. Cobetia sp. QF-1 shows crude oil degradation and biosurfactant production activity at low temperature.
GENOME ANNOUNCEMENT
The Cobetia genus is 1 of 11 genera of the family Halomonadaceae and is most closely related to members of the genus Halomonas (1). The type strain has been described as aerobic, Gram-negative, motile, rod-shaped, and slightly halophilic (2). Cobetia sp. QF-1 can degrade crude oil and produce biosurfactants at 0°C. Although some isolated marine bacteria can degrade oil efficiently around 0°C, most of them have been isolated from polar environments (3, 4); a few have been isolated from China. Several genomes of Cobetia strains have been sequenced before (5–7), but a marine Cobetia strain from China with the capacity to degrade hydrocarbons and produce biosurfactants at 0°C is lacking. Here, we report the draft genome sequence of Cobetia sp. QF-1, isolated from the seawater of Yellow Sea, China.
The genome sequencing of Cobetia sp. QF-1 was performed using the Illumina HiSeq platform at NovogenBio, Beijing, China. Reads were assembled using SOAPdenovo software version 2.21 (8). Protein-coding sequences were functionally annotated with the Clusters of Orthologous Groups of Proteins (COG) (9), Gene Ontology (GO) (10), and KEGG databases (11). tRNAs and rRNAs were detected using tRNAscan-SE (12) and rRNAmmer software (13), respectively.
The genome of Cobetia sp. QF-1 was 4,084,184 bp with a 57.44% GC content. The genome contained 3,513 predicted protein-coding sequences, and the total length of the genes was 3,470,121 bp, which accounted for 84.96% of the genome. The genome also encoded 71 tRNAs and 5 rRNAs.
According to the annotation results, many genes involved in petroleum hydrocarbon degradation, biosurfactant production, and cold adaption were found in the genome of Cobetia sp. QF-1. This draft genome sequence of Cobetia sp. QF-1 will help in understanding the genetic basis of crude oil degradation and biosurfactant production by Cobetia spp.
Accession number(s).
This whole-genome shotgun project has been deposited at DDBJ/ENA/GenBank under the accession number NBVS00000000. The version described in this paper is the first version, NBVS01000000.
ACKNOWLEDGMENT
This work was supported by Fundamental Research Funds for Central Universities, China (grant number 3132015079).
Footnotes
Citation Guo P, Cao B, Qiu X, Lin J. 2018. Draft genome sequence of the crude oil-degrading and biosurfactant-producing strain Cobetia sp. QF-1. Genome Announc 6:e01456-17. https://doi.org/10.1128/genomeA.01456-17.
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