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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Neuropharmacology. 2016 Aug 12;110(Pt A):297–307. doi: 10.1016/j.neuropharm.2016.08.009

Fig. 2.

Fig. 2

Verification of RGS2 protein knockdown via siRNA. N2A-D2R cells were transfected with RGS2 siRNA or corresponding scrambled siRNA. RGS2 protein levels were measured 48 hrs after transfection by Western blotting. (A) Representative Western blot for endogenous RGS2 protein level in control and RGS2 knockdown (RGS2KD) cells probed by mouse anti-RGS2 antibody (SAB1406388, Sigma-Aldrich). (B) Quantification of RGS2 protein levels. RGS2 siRNA knockdown significantly reduced the endogenous RGS2 protein level when compared to scrambled siRNA treated control cells (**P<0.01 vs. control, N=3).