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. Author manuscript; available in PMC: 2018 Jan 22.
Published in final edited form as: Neuron. 2017 Jan 4;93(1):80–98. doi: 10.1016/j.neuron.2016.11.036

Figure 7. BNC-1 and MAB-9 are continuously required to postdevelopmentally maintain motor neuron class identity.

Figure 7

A: Postdevelopmental UNC-3 degradation by treating young adult, conditional unc-3 worms with auxin for 3 days, results in reduction of unc-129 and del-1 expression. All scale bars = 50 µm.

B: Quantification for A; error bars show SD. UNC-3 function is slightly disrupted when fused to AID. Superimposed diagonal stripes indicate dim expression. Unpaired t-tests were performed comparing each auxin-treated condition with its corresponding EtOH control; ***p < 0.001; n ≥ 13. Data of unc-129 and del-1 expression in WT and unc-3 mutants repeated from Fig.2B and Fig.3G, respectively.

C: Postdevelopmental BNC-1 degradation by treating conditional bnc-1 worms at the L4 stage (just before entry into adulthood) with auxin for 1 day results in unc-129 derepression in VA/VB MNs.

D: First-generation progenies (F1) of conditional bnc-1 worms constitutively grown on auxin phenocopy bnc-1 mutants in terms of unc-129 derepression. When auxin is removed at the L4 stage, repression of unc-129 in VA/VB MNs is restored after 3 days.

E: Quantification for C and D; error bars show SD. BNC-1 function is slightly disrupted when fused to AID. Unpaired t-tests were performed comparing each experimental condition with its corresponding untreated control; ***p < 0.001; n ≥ 13.

F: AID-tagged BNC-1 is degraded within 1 h upon auxin treatment. Upon auxin removal, BNC-1 expression is restored within 6 hrs.

G: Postdevelopmental MAB-9 degradation by treating young adult conditional mab-9 worms with auxin for 2 days results in del-1 derepression in DA MNs. MAB-9 function in DB MNs is disrupted non-conditionally when fused to AID.

H: F1 conditional mab-9 worms constitutively grown on auxin phenocopy mab-9 mutants in terms of del-1 derepression. When auxin is removed at the L4 stage, repression of del-1 in DB MNs is not restored even after 4 days, likely due to the hypomorphic nature of this mab-9 allele.

I: Quantification for G and H; error bars show SD. Unpaired t-tests were performed comparing each experimental condition with its corresponding untreated control; ***p < 0.001; n ≥ 13.