FIGURE 4.
Impact of TSA on cell growth and the cell cycle regulator p21Cip1. A, Cell proliferation was evaluated by the MTT assay. Cell viability is expressed as the ratio of the number of viable cells with TSA treatment compared to that without treatment. Cells growing in DMSO were also analyzed as vehicle control. Means (SD) of six replicates are plotted. B, Temporal expression of p21Cip1 (21 kDa); α-tubulin was used as a loading control. The results shown are from 1 representative experiment out of 3 independently performed experiments. MTT, methylthiazolyldiphenyl-tetrazolium bromide.