Table 1. Sequence of primers used in qRT-PCR.
Gene symbol | Accession number | Primer | Primer sequence(5′-3′) | Product size | Tm (°C) |
---|---|---|---|---|---|
IKK | NM_001159774 | Forward | TCAGCTAATGTCCCAGCCTTC | 163 bp | 60 |
Reverse | CCAGTCTAGAGTCGTGAAGC | ||||
IκB | NM204588 | Forward | TGAGGACGAGGACGATAAGC | 146 bp | 60 |
Reverse | ACAACGTGATCGCCATTACCTG | ||||
NF-κB | NM_008689 | Forward | TCAGGAAGAGGTTTGGATGC | 121 bp | 58 |
Reverse | AGCCCCTAATACACGCCTCT | ||||
BcL-2 | NM_009741.5 | Forward | AGCCTGAGAGCAACCCAAT | 159 bp | 61 |
Reverse | AGCGACGAGAGAAGTCATCC | ||||
BcL-xL | NM_009743.5 | Forward | TGTGGATCTCTACGGGAACA | 117 bp | 58 |
Reverse | AAGAGTGAGCCCAGCAGAAC | ||||
Bax | NM_007527.3 | Forward | ATGCGTCCACCAAGAAGC | 163 bp | 61 |
Reverse | CAGTTGAAGTTGCCATCAGC | ||||
Bak | NM007523 | Forward | CGCTACGACACAGAGTTCCA | 175 bp | 57 |
Reverse | CACGCTGGTAGACGTACAGG | ||||
Bim | NM_207680.2 | Forward | GCCAGGCCTTCAACCACTAT | 152 bp | 59 |
Reverse | TGCAAACACCCTCCTTGTGT | ||||
Caspase 3 | NM_009810.3 | Forward | ACATGGGAGCAAGTCAGTGG | 149 bp | 61 |
Reverse | CGTCCACATCCGTACCAGAG | ||||
PARP | NM_007415.2 | Forward | CTCTCCAATCGCTTCTACAC | 108 bp | 57 |
Reverse | GTTGTCTAGCATCTCCACCT | ||||
β-actin | NM_007393 | Forward | GCTGTGCTATGTTGCTCTAG | 117 bp | 60 |
Reverse | CGCTCGTTGCCAATAGTG |