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. 2017 Dec 1;8(70):114698–114709. doi: 10.18632/oncotarget.22815

Figure 4.

Figure 4

(A) Flow cytometry for late apoptosis detection (Annexin V/Propidium iodide) in RPMI8226-LUC-PURO untreated and treated cell line. (B) Flow cytometry for late apoptosis detection (Annexin V/Propidium iodide), in U266-LUC-PURO untreated and treated cell line. Analyzes performed 24 and 48 hours after incubation with bortezomib (100 nM) or VER155008 (50 μM or 80 μM) or bortezomib plus VER155008 (50 μM or 80 μM) (named as COMB50 μM or COMB80 μM) for both cell lines. X-axis: time, Y-axis: percentage of cells labeled with annexin V + and PI +. Experiments were performed in triplicates. Two-Way ANOVA, with Bonferroni’s post -test.