Figure 7. Validation of differentially expressed lncRNAs in silica particle exposed macrophages.

In GSE13005 microarray data set, the log₂ folded change value of each differentially expressed lncRNA was shown in red color with a solid line, refering to the left Y axis. The up/down regulated lncRNAs were divied by the solid line (Y = 0), lncRNA Meg3 (A) and Rain (B) were up regulated (p < 0.01), while Pvt1 (C), 1700020I14Rik (D), and 2900097C17Rik (E) were down regulated (p < 0.01). Consistently, the lncRNAs involved in macrophages that exposed to a concentration of 100 μg/ml silica particles (3 samples in parallel per lncRNA) shown the same expression trend as RNA-Seq (the p valves of A–E were < 0.001, < 0.001, 0.002, 0.003, and < 0.001 respectively), and the relative expression levels of the selected lncRNAs were shown in blue color with a dotted line (right Y axis, Y = 1).