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. 2017 Dec 12;6:e32027. doi: 10.7554/eLife.32027

Figure 3. Selective labeling of PNs that receive synaptic input from ORNs in identified glomeruli.

Figure 3.

(a) Labeling of PNs that receive synaptic input from ORNs expressing the ligand nSyb::CD19 in identified glomeruli using the R17H02 (top panels) and R28H10 (bottom panels) LexA drivers. Left: nSyb::CD19+ axons from ORNs (red) driven by R17H02- and R28H10-LexA branch in identified glomeruli (stippled circles). In R17H02 the nSyb::CD19 expression level was low in VA6 and VA1lm, and expression in DA1 was only visible after signal amplification by immunostaining. Center: Induction of CD4::tdGFP expression in PNs triggered by nSyb::CD19+ ORNs (red, left panels). In R17H02 (top center) two uniPNs with dendrites branching into DA1 (arrows) and one neuron branching into VA6 (arrowhead) were labeled. No GFP+ PN branched in VA1lm. In R28H10 (bottom center) a single uniPN with dendrites branching in VC1 was GFP+ (arrow). (b) Tracing the neuronal connections from the ORNs expressing the ligand CD19::sdc in identified glomeruli by using the R17H02 (top panels) and R28H10 (bottom panels) LexA drivers. Left: CD19::sdc+ axons from ORNs (red) driven by R17H02- and R28H10-LexA branch in the identified glomeruli (stippled circles). In the VA6 glomerulus of R17H02 and VC1 of R28H10, the CD19::sdc expression level was too low to be detected by immunostaining, but the expression in DA1 and VA1lm of R17H02 was above the detection level. Center: Induction of CD4::tdGFP expression in PNs triggered by CD19::sdc+ ORNs (red, left panels). In R17H02 (top center) there were uniPNs projecting to DA1 (arrows) and VA6 (arrowhead). In R28H10 (bottom center) a single uniPN projecting to VC1 was GFP+ (arrow). Expression of CD19::sdc with R28H10 and R17H02 induced GFP expression in a single multiPN (yellow arrowhead). (c) The diagrams show the induction pattern of uniPNs from (a) and (b). The multiPNs detected in (b) are not included. Scale bar = 50 μm.