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. 2017 Dec 5;9(1):21–36. doi: 10.18632/oncotarget.22940

Figure 1. The senescence-responsive region of the OPN promoter contains c-Myb and C/EBPβ binding sites.

Figure 1

a. Treatment of BJ fibroblasts with bleomycin induces a significant increase in senescence as indicated by increased senescence-associated β-galactosidase staining, n=3, *p<0.05. Scale bar=100 μm b. Schematic of expression of luciferase reporter constructs that were driven by fragments of the OPN promoter spanning from nucleotide +86 to the indicated number of bases upstream from the transcription start site (TSS; top). BJ fibroblasts expressing the indicated promoter reporter constructs were treated with vehicle or bleomycin to induce senescence. Relative luciferase activity in senescent relative to non-senescent fibroblasts indicates a senescence-responsive element (SRE) between -135 and -190 bases upstream of the TSS (bottom), n=3. c. Schematic of OPN promoter. The SRE includes putative binding sites for the transcription factors c-Myb and C/EBPβ.