Table 2.
Serum hemagglutination inhibition (HI) and enzyme-linked lectin assay (ELLA) antibody titers following NA VLP vaccination in ferretsa.
| Vaccine group | HI titerb
|
ELLA titerb
|
||
|---|---|---|---|---|
| Pre-boost | Pre-challenge | Pre-vaccine | Pre-challenge | |
| Experiment 1 | ||||
| H5/N1/M1 | 113 (80–160) | 226 (160–320) | 5 (5) | 10240 (10240) |
| H3/N1/M1 | < 10 | < 10 | 5 (5) | 905 (640–1280) |
| H3/N2/M1 | < 10 | < 10 | 5 (5) | 47.6 (20–80) |
| N1/M1 | < 10 | < 10 | 5 (5) | 3620 (2560–10240) |
| PBS | < 10 | < 10 | 5 (5) | 5 (5) |
| Experiment 2 | ||||
| H5/N1/M1 | 135 (80–160) | 320 (320) | 14.1 (5–20) | 4305 (2560–5120) |
| H3/N1/M1 | < 10 | < 10 | 11.9 (5–20) | 403.2 (320–640) |
| H3/N2/M1 | < 10 | < 10 | 17.4 (10–20) | 30.3 (20–40) |
| N1/M1 | < 10 | < 10 | 10 (5–20) | 2153 (1280–2560) |
a
Ferrets were intramuscularly vaccinated twice (4–5 weeks apart).
b
Samples were tested for HI and ELLA antibody activity against A/Indonesia/05/2005 virus. Geometric mean titers (range in parentheses) are shown as the dilution of serum that inhibits agglutination of horse or turkey RBC’s or that exhibits ≥ 50% inhibition of NA enzymatic activity.