Figure 1.

FATp300 inhibitor L002 reduces differentiation, migration and proliferation of cardiac fibroblasts. (A to E) Human cardiac fibroblasts (HCFs) or rat cardiac fibroblasts (RCFs) were cultured and treated in triplicate with L002 or C646 or DMSO in the presence or absence of TGF-β. Total protein were isolated, pooled and processed for Western blot (A, C, D) using indicated antibodies. Experiments were repeated two times. Total RNA were isolated and processed for qPCR in triplicate (B, E) using gene specific primers. (F) For migration study, the scratch wounds were made in monolayer cultures of HCFs. Cells were then pretreated in triplicate with L002 or DMSO for 1 hour followed by TGF-β treatment for 24 h. Photographs were taken at 0 hour and 24-hour post-treatment. (G) For proliferation study, HCFs were pretreated in triplicate with L002 or DMSO for 1 h followed by treatment with TGF-β. Cell numbers were counted at 24 h and 48 h. Data represented as mean ± SEM. **p < 0.01, ***p < 0.005.