Figure 4.

Effects of the U1 deletion on the expression levels of the Peg3 domain. Potential effects of the U1 deletion on the expression levels of the individual genes were analyzed using a set of total RNA isolated from neonatal heads (A) and adult hypothalamus (B) of WT and KO(−/+) mice with maternal transmission of the deletion. This series of qRT-PCR analyses tested four imprinted genes, Peg3, Usp29, Zim1, and Zfp264. The expression levels of each gene were first normalized with an internal control (β-actin), and the subsequent value was compared between KO and WT. The relative levels are presented with their standard deviation. This set of analyses was repeated using two biological replicates.