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. 2017 Dec 12;12(10):875–885. doi: 10.1080/15592294.2017.1361592

Table 3.

Primer information.

Gene Primer sequence CpG Coverage Location CpG loci
CYP1A1 F: 5′- Biotin-GGTTTTTGTTTGAGAGGAGAGGTAGTT-3′ 6 Chr15: 74,726,798 -
promoter R: 5′- ACAACTTTTTTTTTCCTAAAAACCCTAT-3′   Chr15:74,726,863
  S: 5′- ACCCTATAACAAAAAAATTC-3′    
AHRR F: 5′- GGATATAGGGGTTGTTTAGGTTATAGA-3′ 3 Chr5: 373,464 -
body R: 5′- Biotin-AAAAAACCCTACCAAAACCACTCC -3′   Chr5: 373,509
  S: 5′- GTTTTGGTTTTGTTTTGTATT -3′    
AHRR Qiagen assay Hs_PDCD6_03_PM 5 Chr5: 322,640 -
promoter     Chr5: 322,662
GALNT2 F: 5′- GTGGGTAGTAATTTGTGTTTGGATAG -3′ 3 Chr1: 230,279,439 -
body R: 5′- Biotin-TATAATCACCAAACTCCACCCAATAA -3′   Chr1: 230,279,479
  S: 5′- GGTTTTTTYGTAGTAGTGGAAG-3′    
GALNT2 F: 5′-Biotin- GGTRGAGTTGGGAGAATG-3′ 5 Chr1:230,067,284-
promoter R:5′- AACRCTCCACTCACCTTCCTACC-3′   Chr1:230,067,298
  S: 5′- CAAAAAAACRAAACAAAACAACATC-3′    
GFI1 F: 5′- Biotin- TGGGGTTAGAGAGAAGGT -3′ 4 Chr1:92,480,576-
body R: 5′- ACCACCRCTAACCCTAACCTAAAACTCTA -3′   Chr1:92,480,634
  S: 5′- ACTATACACCCRCCTACTACTAAA -3′    
GFI1 F: 5′- GGTAGGGRGGAGTTAGTTTAGTAG-3′ 10 Chr1:92,474,769-
promoter R: 5′-Biotin- CCCAATCCRAAAAATTTACTCATTTCCC-3′   Chr1:92,474,805
  S: 5′-GAGTTTRGRGTAGGGAGGG-3′    
RUNX3 F: 5′- Biotin- GATGGGTTTTGGGAATTAGAGTTTAAG-3′ 1 Chr1:25,342,715
body R: 5′- ACTAACATAACCCCRAAATAATACATCCTA-3′    
  S: 5′- AAAAAAAAATCAATTCCAACT-3′    
RUNX3 F: 5′- AGTTTRGGGAGTAGTGGGGATG-3′ 7 Chr1:24,935,146 -
promoter R: 5′- Biotin-ACAACCCCRTTAAAAATCATTCCTACA-3′   Chr1:24,935,173
  S: 5′- GAGTAGTGGGGATGG-3′    
TSLP F: 5′- AAGGGTTTTTTGTGGATTGGTAA -3′ 1 Chr 5:111,071,845
body R: 5′-Biotin- CAATTCCACCCCAATTTCACACT -3′    
  S: 5′- TTTAAGGTAGGTTTTATAGATTTT -3′    
TSLP F: 5′- GGAGGAAAGGTAAATTGGGAATTG-3′ 6 Chr 5:111,073,649-
promoter R: 5′-Biotin- CTCRCTCCTCCCAAATCTCCAAAA-3′   Chr 5:111,073,670
  S: 5′- GGAARGTTGTTAGGGGTATTATT-3′