Figure 1.

Autophagy is an active process during germ cell differentiation in moss. (A) Schematic representation of exposed reproductive organs in a gametophore apex at the moment of fertilization (left) and different stages of antheridium and archegonium development as described previously.²³ (B) Representative epifluorescence pictures of WT reproductive organs expressing GFP-PpATG8e from the PpATG8e promoter in a cell- and stage-specific manner. GFP signals are shown in green, while red is chlorophyll autofluorescence. The red signal marks primarily the jacket cells in antheridia and the neck or body cells in archegonia. Due to large organ size, some archegonia images have been mounted from 2 complementary pictures.