Table 1. Strains with telomere dysfunction have increased rates of white colony formation.
TER1 allele | Telomere length | Total colonies observed | White colonies observed | Percentage of white colonies (%) | Median |
---|---|---|---|---|---|
Wild-type | 400–600 bp | 57,983 | 0 | <0.002 | 0.00% (18) |
ter1-Δ | Variable length >100 bp at senescence | 32,484 | 1560 | 4.80 | 11.49% (27) |
stn1-M1 | Very long and heterogenous | 55,272 | 173 | 0.31 | 1.30% (22) |
stn1-M1 ter1Δ | Very long and heterogenous | 78,187 | 797 | 1.02 | 2.44% (18) |
taq-28C | 75–150 bp | 134,375 | 90 | 0.07 | 0.02% (30) |
Dilution plates were examined for white colony formation in seven strains with varying levels of telomere function. The number of independent isolates used to generate the median frequency of white colonies is indicated in parentheses in the median column. Median frequency of white colonies formed was calculated from the average number of white colonies formed across the different independent isolates (see Materials and Methods for detailed description).