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. 2017 Dec 12;208(2):565–578. doi: 10.1534/genetics.117.300606

Figure 1.

Figure 1

Tension-sensing motif (TSM) function is modulated by lysine residues within the histone H3 tail domain in an SGO1-dependent manner. (A) Strains with the listed histone H3 alleles were transformed with empty vector (EV) or overexpression plasmids for either wild-type (WT) or a catalytically inactive E173H Gcn5p. Logarithmically growing cultures were serially diluted and spotted to YPD or YPD containing 10 μg/ml benomyl. (B) Strains with histone H3 K9, 14, 18, and 23 mutated to alanine (4K-A) along with a WT or G44S TSM allele were assessed by spot assay as described above. (C) The 4K-A mutations were tested in ∆sgo1 strains to assess the SGO1 dependence of the 4K-A rescue.