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. 2017 Dec 12;208(2):565–578. doi: 10.1534/genetics.117.300606

Figure 5.

Figure 5

K14A rescues aberrant mating phenotypes of diploid tsm− strain. Diploid strains containing the wild-type (WT), K14A, G44S, and K14A G44S alleles as the sole copy of H3 were subjected to mating with haploid tester strains. Colony formation on minimal medium indicates aberrant mating resulting from aneuploidy. (A) Representative images of colonies on minimal medium plates. Arrows indicate colony growth. (B) Colony growth shown by box plot. Data represents three independent biological replicates, and a total of 120 patches of each strain analyzed. Both WT and K14A strains show low levels of mating, which are greatly increased in the G44S strain. This aberrant mating can be reduced with the introduction of K14A into the G44S strain.