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. 2017 Dec 20;9(2):1542–1552. doi: 10.18632/oncotarget.23457

Figure 2. The effect of B7-H3 on mouse SSC proliferation.

Figure 2

A. The expression of C-kit and Oct-4 on mouse SSCs was analyzed by flow cytometry. B. Mouse SSCs were incubated with different concentrations of B7-H3 (0, 5, 10, 25 ng/mL) for various time points (1, 6, 12, 24, 48, 72 h) in vitro. Then, cells were collected for proliferation assessment using CCK8 assays. The results are expressed as the mean±standard deviation (n = 3). Differences were analyzed by two-way ANOVA followed by Tukey's post hoc analysis. ###P < 0.001 versus 24 h. **P < 0.01, ***P < 0.001 versus 0 ng/ml. ▲P < 0.05, ▲▲▲P < 0.0001 versus 5 ng/ml. C. to F. Representative images of SSCs incubated with different concentrations of B7-H3 at 48 h. G. The SSCs were co-cultured with TM4 for 6 to 72 h in vitro, and the supernatants were collected at each time point for B7-H3 evaluation. The data were analyzed by repeated measures one-way ANOVA. H. The co-cultured SSCs in (G) were harvested and assessed for proliferation as described in (B). Data were analyzed by repeated measures one-way ANOVA with Tukey's post tests. #P < 0.05 versus 24 h.