In Table 2, the names and sequences listed for Cinnamon_2psb-for and Cinnamon_2trnH-rev are incorrect. The sequence listed for trnHf is also incorrect. Please see the corrected Table 2 here.
Table 2. Primers used in the present study for DNA barcoding analyses.
Locus | Primer name | Sequence (5’-3’) | Ta | Primer note | Reference |
---|---|---|---|---|---|
rbcL | rbcLa_F | ATG TCA CCA CAA ACA GAG ACT AAA GC | 55 | Universal | [35] |
rbcLajf634R | GAA ACG GTC TCT CCA ACG CAT | Universal | [36] | ||
CS_rbcL-300rev | TAA AGG ATA CGC TAC ATA AGC | 55 | Specific for Camellia sinensis; used to check the SNP-M (68 bp) in sequencing PCR | In the present study | |
matK | 1R_KIM-f | ACC CAG TCC ATC TGG AAA TCT TGG TTC | 55 | Universal | Ki-Joong Kim, pers. comm. |
3F_KIM-r | CGT ACA GTA CTT TTG TGT TTA CGA G | ||||
trnH(GUG)-psbA | psbA3'f | GTT ATG CAT GAA CGT AAT GCT C | 55 | Universal | [37] |
trnHf* | CGC GCA TGG TGG ATT CAC AAT CC | [38] | |||
Can_3psb-for | GTT TCT TCT GTA CCA ACC TGC | 56 | Specific for Cinnamomum verum and C. cassia (cinnamon) | In this study | |
Can_3trnH-rev | TAC TTC ACT CTC CTT CCT GCT | ||||
Citrus_psbA-in | GTA TTG ATC CGT TAT TTA GTC G | 57 | Specific for Citrus sp. (lemon, orange, grapefruit, and lime) | In this study | |
Citrus_trnH-in | ATC TTA TCT TAC TTA TGA AGA ACC | ||||
Glycyrrhiza_psbA-in | GTT TTA AAG AAG GAT ACG AGG | 55 | Specific for Glycyrrhiza glabra (licorice) | In this study | |
Glycyrrhiza_trnH-inA | TAC ATT CGC CCT TCT TAT AC | ||||
Mentha_2psb_for | TTC CAG GCA AGT CCA ATA CT | 56 | Specific for Mentha sp. (M. aquatica, M. arvensis, M. canadensis, M. longifolia, P. pulegium, M. suaveolens, M. pulegium, and M. x piperita, M. spicata) | In this study | |
Mentha_2trnH_rev | TGT GTA GGA GTT TTT GAA AAT AGA C | ||||
Piminella_psbA-in | ACC TAG CTG CTG TTG AAG CTC | 60 | Specific for Pimpinella anisum (anise) | In this study | |
Pimpinella_trnH-in | GGA GCA ATA TCG CTT TCT TGA TAG A | ||||
rps7-trnV(GAC) | CS_rps7_1 | GTG CTA TGG CTC GAA TCC GT | 67 | Specific for Camellia sp.; used to discriminate C. sinensis from C. pubicosta | In this study |
CS_trnV(GAC)_1* | ACC ACG TCA AGG TGA CAC TC | ||||
P6 loop trnL(UAA) | g | GGG CAA TCC TGA GCC AA | 55 | Universal | [39] |
h* | CCA TTG AGT CTC TGC ACC TAT C | ||||
ITS2 | S2F | ATG CGA TAC TTG GTG TGA AT | 55 | Universal | [40] |
S3R* | GAC GCT TCT CCA GAC TAC AAT | ||||
BELL-1 | GGD GCG GAK AHT GGC CYC CCG TGC | 55 | Universal | [41] | |
BELL-3 | GAC GCT TCT CCA GAC TAC AAT |
*, labeled
Reference
- 1.De Castro O, Comparone M, Di Maio A, Del Guacchio E, Menale B, Troisi J, et al. (2017) What is in your cup of tea? DNA Verity Test to characterize black and green commercial teas. PLoS ONE 12(5): e0178262 https://doi.org/10.1371/journal.pone.0178262 [DOI] [PMC free article] [PubMed] [Google Scholar]