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. 2018 Jan 30;13(1):e0192334. doi: 10.1371/journal.pone.0192334

Correction: What is in your cup of tea? DNA Verity Test to characterize black and green commercial teas

Olga De Castro, Maria Comparone, Antonietta Di Maio, Emanuele Del Guacchio, Bruno Menale, Jacopo Troisi, Francesco Aliberti, Marco Trifuoggi, Marco Guida
PMCID: PMC5790277  PMID: 29381771

In Table 2, the names and sequences listed for Cinnamon_2psb-for and Cinnamon_2trnH-rev are incorrect. The sequence listed for trnHf is also incorrect. Please see the corrected Table 2 here.

Table 2. Primers used in the present study for DNA barcoding analyses.

Locus Primer name Sequence (5’-3’) Ta Primer note Reference
rbcL rbcLa_F ATG TCA CCA CAA ACA GAG ACT AAA GC 55 Universal [35]
rbcLajf634R GAA ACG GTC TCT CCA ACG CAT Universal [36]
CS_rbcL-300rev TAA AGG ATA CGC TAC ATA AGC 55 Specific for Camellia sinensis; used to check the SNP-M (68 bp) in sequencing PCR In the present study
matK 1R_KIM-f ACC CAG TCC ATC TGG AAA TCT TGG TTC 55 Universal Ki-Joong Kim, pers. comm.
3F_KIM-r CGT ACA GTA CTT TTG TGT TTA CGA G
trnH(GUG)-psbA psbA3'f GTT ATG CAT GAA CGT AAT GCT C 55 Universal [37]
trnHf* CGC GCA TGG TGG ATT CAC AAT CC [38]
Can_3psb-for GTT TCT TCT GTA CCA ACC TGC 56 Specific for Cinnamomum verum and C. cassia (cinnamon) In this study
Can_3trnH-rev TAC TTC ACT CTC CTT CCT GCT
Citrus_psbA-in GTA TTG ATC CGT TAT TTA GTC G 57 Specific for Citrus sp. (lemon, orange, grapefruit, and lime) In this study
Citrus_trnH-in ATC TTA TCT TAC TTA TGA AGA ACC
Glycyrrhiza_psbA-in GTT TTA AAG AAG GAT ACG AGG 55 Specific for Glycyrrhiza glabra (licorice) In this study
Glycyrrhiza_trnH-inA TAC ATT CGC CCT TCT TAT AC
Mentha_2psb_for TTC CAG GCA AGT CCA ATA CT 56 Specific for Mentha sp. (M. aquatica, M. arvensis, M. canadensis, M. longifolia, P. pulegium, M. suaveolens, M. pulegium, and M. x piperita, M. spicata) In this study
Mentha_2trnH_rev TGT GTA GGA GTT TTT GAA AAT AGA C
Piminella_psbA-in ACC TAG CTG CTG TTG AAG CTC 60 Specific for Pimpinella anisum (anise) In this study
Pimpinella_trnH-in GGA GCA ATA TCG CTT TCT TGA TAG A
rps7-trnV(GAC) CS_rps7_1 GTG CTA TGG CTC GAA TCC GT 67 Specific for Camellia sp.; used to discriminate C. sinensis from C. pubicosta In this study
CS_trnV(GAC)_1* ACC ACG TCA AGG TGA CAC TC
P6 loop trnL(UAA) g GGG CAA TCC TGA GCC AA 55 Universal [39]
h* CCA TTG AGT CTC TGC ACC TAT C
ITS2 S2F ATG CGA TAC TTG GTG TGA AT 55 Universal [40]
S3R* GAC GCT TCT CCA GAC TAC AAT
BELL-1 GGD GCG GAK AHT GGC CYC CCG TGC 55 Universal [41]
BELL-3 GAC GCT TCT CCA GAC TAC AAT

*, labeled

Reference


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