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. 2017 Dec 14;7(3):e1408750. doi: 10.1080/2162402X.2017.1408750

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Figure 1. — Melanoma growth induces immune cell dysregulation, anemia and thrombocytopenia: (A) C57 BL/6 mice were subcutaneously injected with 2 × 10⁵ B16-F10 melanoma cells. Mice were euthanized at 21 days post tumor implantation (D21) and blood, spleen and BM was analyzed. No tumor (NT) mice were used as controls. Blood cells analyzed are shown. (B, C and D) White blood cell (WBC) counts, Hemoglobin (HB), hematocrit (HCT), RBC and platelet counts in peripheral blood are shown. Last graph in (C) shows percentage of B cells, CD4 and CD8 T lymphocytes in the blood as analyzed by flow. Data was analyzed by Student's t-test. Mean ± SD are shown. *p < 0.05, ***p < 0.005, ****p < 0.001.