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. 2017 Dec 17;9(3):2984–3000. doi: 10.18632/oncotarget.23354

Figure 5. Synergistic induction of cell death by a combination of low dose BZ with JNK-IN-8.

Figure 5

A. HMC-1.2 cells were pre-incubated with vehicle (DMSO) or JNK-IN-8 for 1h followed by TM (10µg/ml) or BZ (10 nM) treatment for 24h. Phosphorylation of JNK and JUN was detected by Western blotting. GAPDH served as loading control. (n=3) B. HMC-1.2 cells were pretreated for 1h with vehicle (DMSO) or JNK-IN-8 (3µM) followed by a 24h BZ treatment. JUN mRNA expression was evaluated by RT-qPCR and normalized to HPRT. (n=4) C, D. HMC-1.2 cells were treated with DMSO or 3µM JNK-IN-8 for 24h and proliferation (Casy cell counting) C (n=4) and metabolic activity (XTT assay) D (n=3) were measured. E. Cell viability was determined by FACS analysis of Annexin V/ propidium iodide stained HMC-1.2 cells treated for 72h with the indicated substances. One representative experiment is shown. F. Statistical analysis of the amount of apoptotic cells after 72h of independent experiments as measured in E. (n=3) Data shown are mean ± SD from n ≥ 3 independent experiments. Student’s t-Test and one-sample t-Test were performed to calculate the p-values. *p < 0.05, **p < 0.01, ***p < 0.001.